SARS-CoV-2 Neutralizing Antibody Assay Kit (DEIASL055)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
Serum, plasma
Species Reactivity
Human
Intended Use
The RBD Neutralizing Antibody competitive ELISA Kit is an enzyme-linked immunosorbent assay intended for the qualitative detection of an RBD neutralizing antibodies in serum or plasma.
Storage
1. The test kit has to be stored at a temperature between +2°C and +8°C, do not freeze, and avoid exposure to direct sunlight. The unopened Is stable for at least 10 months. The opened kit can be stored at a temperature between +2°C and +8°C for 2 weeks.
2. The opened positive control can be stored at a temperature between+2°C and +8°C for 7 days. For longer storage, please aliquot and freeze at-20°C for no longer than 3 months, avoid repeated freeze-thaw cycles.
Sensitivity
The cut-off value was determined at 99% sensitivity of 1735 negative sera, as < 20%.
General Description
S protein contains a receptor-binding domain(RBD) which is one of the vital immunodominant epitopes and has a superior capacity to induce neutralizing antibodies. It is proved that RBD is responsible for recognizing and interacting with he cell surface receptor, angiotensin-converting enzyme-2 (ACE2). Following the binding of the RBD to the receptor ACE, pathogen enters target cells, where the fusion of the virus envelops the endosome membranes and leads to the release of the viral nucleocapsid into the cytosol of the infected cell.

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