This kit is intended for the quantitative detection of the recombinant SARS-COV-2 nucleoprotein antigen in human serum. It can recognize recombinant SARS-CoV Nucleoprotein / NP Protein, but no react with recombinant MERS-CoV Nucleoprotein / NP protein. The use of this kit for natural samples need be validated by the end user due to the complexity of natural targets and unpredictable interference.
The kit should be stored at 2 ~ 8 °C, protected from light, and valid for 6 months.
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.