Rat PAI-1 Total Antigen ELISA Kit

Sample

Plasma

Intended Use

This rat plasminogen activator inhibitor type 1 (PAI-1) total antigen assay is intended for the quantitative determination of total PAI-1 in biological fluids. For research use only.

Storage

Store all kit components at 4°C upon arrival. Return any unused microplate strips to the plate pouch with desiccant. Reconstituted standard and primary may be stored at -80°C for later use. Do not freeze-thaw the standard and primary antibody more than once. Store all other unused kit components at 4°C. This kit should not be used beyond the expiration date.

Sensitivity

The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.123-0.132) and calculating the corresponding concentration. The MDD was 0.047ng/mL.

General Description

Plasminogen activator inhibitor-1 (PAI-1) is a central regulator of the blood fibrinolytic system [1]. Clinical studies have indicated that increased PAI-1 levels increase the risk for thrombosis, whereas decreased levels may cause recurrent bleeding [2].