Human Serpin Peptidase Inhibitor, Clade E (nexin, plasminogen activator inhibitor type 1), Member 1, SERPINE1 ELISA Kit (CKERS-SERPINE1-209H)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma, other biological fluids
Species Reactivity
Human
Intended Use
This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). It is developed for quantitative measurement of Human SERPINE1 in serum, plasma and other biological fluids.
Contents of Kit
1. Human SERPINE1 Microplate: polystyrene microplate coated with a monoclonal antibody against Human SERPINE1;
2. Standard (freeze dried): 10 ng/mL, 2 vials;
3. Biotin-antibody (100x): 2 vials;
4. HRP-avidin (100x): 2 vials;
5. Reagent Diluent: 25 mL, 2 vials;
6. TMB Substrate: 12 mL, 1 vial;
7. TMB Stop Solution: 1 vial;
8. Wash Buffer (20x): 30 mL, 1 vial;
9. Microtiter plate sealers: 3 sheets.
Storage
Unopened Kit: Store at 2-8°C. Do not use past kit expiration date.
Opened/Reconstituted Reagents: Standard or Sample Diluent; Biotin-antibody Diluent; HRP-avidin Diluent; TMB Substrate; Wash Buffer; TMB Stop Solution. The above mentioned reagents should be stored for up to 1 month at 2-8°C.
Microplate Wells: Return unused wells to the foil pouch containing the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2-8°C.
Sensitivity
Detection Rage: 0.156-10 ng/mL.
Detection Limit: The minimum detectable dose of Human SERPINE1 is typically less than 0.07 ng/mL.

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References


Divella, R; Mazzocca, A; et al. Influence of Plasminogen Activator Inhibitor-1 (SERPINE1) 4G/5G Polymorphism on Circulating SERPINE-1 Antigen Expression in HCC Associated with Viral Infection. CANCER GENOMICS & PROTEOMICS 9:193-198(2012).
Sotos-Prieto, M; Guillen, M; et al. Association between the rs6950982 polymorphism near the SERPINE1 gene and blood pressure and lipid parameters in a high-cardiovascular-risk population: interaction with Mediterranean diet. GENES AND NUTRITION 8:401-409(2013).

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