Active Rat PAI-1 ELISA Kit

Sample

Plasma, serum

Intended Use

This rat plasminogen activator inhibitor type 1 (PAI-1) activity assay is intended for the quantitative determination of active PAI-1 in rat plasma and serum. For research use only.

Storage

Store all kit components at 4°C upon arrival. Return any unused microplate strips to the plate pouch with desiccant. Reconstituted standard and primary may be stored at -80°C for later use. Do not freeze-thaw the standard and primary antibody more than once. Store all other unused kit components at 4°C. This kit should not be used beyond the expiration date.

Sensitivity

The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.064-0.071) and calculating the corresponding concentration. The MDD was 0.032ng/mL.

General Description

Plasminogen activator inhibitor-1 (PAI-1) is a serine protease inhibitor that is an important regulator of fibrinolysis and extracellular matrix turnover [1,2,6]. PAI-1 may be important in hepatocyte growth and proliferation in vivo. Increased PAI-1 levels may increase the risk for myocardial infarction, atherosclerosis, and retenosis [3,4]. Increased PAI-1 levels may also play an important role of the development and pathogenesis of diabetic nephropathy [5]. Decreased levels may reduce thrombotic events [7].