Q: Could you let me know the application for this kit?
A: In a 1:10 ratio of collection media to blood. The sample size is 100uL.
Q: PAI standard: What is the amount of standard in your kit? How to dilute the standard?
A: Add 5ml blocking buffer to get 50ng/ml stocking solution. The amount is 250ng, S0(Blocking Buffer) 0, S1 0.05, S2 0.1ng/ml, S3 0.25ng/ml, S4 0.5ng/ml, S5 1ng/ml, S6 2.5ng/ml, S7 5ng/ml, S8 10ng/ml, S9 25ng/ml, S10 50ng/ml
Q: What is the amount of the antibody in this kit? How to dilute?
A: PAI-1 Antibody(Primary antibody)- add 10ml blocking buffer. Enzyme conjugate Antibody(Secondary antibody) - dilute 1ul into 10ml blocking buffer.
COA
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