Established by fusion of the P3X62AG8.653 mouse myeloma cell line with spleen cells of a BALB/c mouse immunized with Epstein-Barr virus (EBV) particles; cells produce a monoclonal antibody (igg1) that reacts with a viral envelope glycoprotein (350/220 kd) and that neutralizes EBV confirmed as mouse with IEF of AST, MDH, PEP B. . Viruses: ELISA: reverse transcriptase positive; PCR: SMRV
Morphology
single cells in suspension
Growth Properties
suspension
CultureMethod
Subculturing
Split ratio: 1:3 to1: 5, every 2-3 days; seed out at 0.1-1.0 x 10^6 cells/ml; Harvest: maximal density of about 1.0-1.5 x 10^6 cells/ml.
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COA
Publication ![The purity of recombinant EBV [His] >95%, determined by SDS-PAGE](https://resource.creative-diagnostics.com/diagnostics/productimages-1/DAG1578-1.jpg)
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