Recombinant EBV p18 (DAGA-3074)

EBV p18, Recombinant protein from E.coli for WB, ELISA

Product Overview
The E.Coli derived recombinant mosaic protein contains the HHV-4 p18 regions, 1-119 amino acids.
Nature
Recombinant
Tag/Conjugate
TBD
Alternative Names
Epstein Barr Virus; EBV; Epstein–Barr virus; human herpesvirus 4; HHV-4; Human herpesvirus 4
Purity
>95% , based on SDS PAGE
Format
Each vial contains 100 µg of lyophilized protein in 50mM Tris-HCl pH 8.0, 60mM NaCl and 50% glycerol. Reconstitute the protein with 100 µl of Millipore water.
Concentration
Batch dependent - please inquire should you have specific requirements.
Size
100ug
Storage
Before reconstitution, stable for 1 year at -20 centigrade from the date of shipment. After reconstitution, stable for a month at 4 centigrade. Nonhazardous. No MSDS required.

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References


A case of poorly differentiated adenocarcinoma with lymphoid stroma originated in the ascending colon diagnosed as lymphoepithelioma-like carcinoma

CLINICAL JOURNAL OF GASTROENTEROLOGY

Authors: Kai, Kengo; Hidaka, Hideki; Nakamura, Takeshi; Ueda, Yuji; Marutsuka, Kosuke; Ikeda, Takuto; Nanashima, Atsushi

An 86-year-old woman's stool sample was positive for blood. Computed tomography (CT) showed wall thickening of the ascending colon at the hepatic flexure. Colonoscopy showed near-complete obturation by colon cancer. Since she was asymptomatic, elective surgery was planned. Laparoscopic right hemicolectomy was performed. Histopathological examination showed poorly differentiated carcinoma cells proliferating in a solid pattern with marked lymphocyte infiltration. The diagnosis was lymphoepithelioma-like carcinoma (LELC) associated with Epstein-Barr virus (EBV) infection; however, EBV-encoded small RNA-in situ hybridization was negative. Microsatellite instability was not assessed. The postoperative course was uneventful and she was discharged on the 15th postoperative day. She remains recurrence-free at 2 years after surgery. Past reports note that colorectal carcinomas with dense lymphoid stroma may be related to LELC or medullary carcinoma (MC). Gastrointestinal LELC is rare, with some reports on LELC of the esophagus and stomach. Reports on LELC of the large intestine are very rare. MC of the large intestine is relatively new concept, firstly described in the WHO Classification of Tumours of the Digestive System 3rd Edition in 2000. We herein present a case of lymphoepithelioma-like carcinoma of the ascending colon and relevant case reports about LELC and MC of the large intestine.

Status of kinases in Epstein-Barr virus and Helicobacter pylori Coinfection in gastric Cancer cells

BMC CANCER

Authors: Sonkar, Charu; Verma, Tarun; Chatterji, Debi; Jain, Ajay Kumar; Jha, Hem Chandra

BackgroundHelicobacter pylori (H. pylori) and Epstein - Barr virus (EBV) plays a significant role in aggressive gastric cancer (GC). The investigation of genes associated with these pathogens and host kinases may be essential to understand the early and dynamic progression of GC.AimThe study aimed to demonstrate the coinfection of EBV and H. pylori in the AGS cells through morphological changes, expression of the kinase and the probable apoptotic pathways.MethodsGenomic DNA isolation of H. pylori and its characterization from clinical samples were performed. RT-qPCR of kinases was applied to scrutinize the gene expression of kinases in co-infected GC in a direct and indirect (separated through insert size 0.45 mu m) H. pylori infection set up. Morphological changes in co-infected GC were quantified by measuring the tapering ends of gastric epithelial cells. Gene expression profiling of apoptotic genes was assessed through RT-qPCR.ResultsAn interleukin-2-inducible T-cell kinase (ITK) showed significant upregulation with indirect H. pylori infection. Moreover, Ephrin type-B receptor six precursors (EPHB6) and Tyrosine-protein kinase Fyn (FYN) showed significant upregulation with direct coinfection. The tapering ends in AGS cells were found to be extended after 12h. A total of 24 kinase genes were selected, out of which EPHB6, ITK, FYN, and TYK2 showed high expression as early as 12h. These kinases may lead to rapid morphological changes in co-infected gastric cells. Likewise, apoptotic gene expression such as APAF-1 and Bcl2 family genes such as BAD, BID, BIK, BIM, BAX, AND BAK were significantly down-regulated in co-infected AGS cells.ConclusionAll the experiments were performed with novel isolates of H. pylori isolated from central India, for the functional assessment of GC. The effect of coinfection with EBV was more profoundly observed on morphological changes in AGS cells at 12h as quantified by measuring the tapering of ends. This study also identifies the kinase and apoptotic genes modulated in co-infected cells, through direct and indirect approaches. We report that ITK, EPHB6, TYK2, FYN kinase are enhanced, whereas apoptotic genes such as APAF-1, BIK, FASL, BAX are significantly down-regulated in AGS cells coinfected with EBV and H. pylori.

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