EBV p18 protein [GST] (DAG1580)

EBV [GST], recombinant protein from E. coli

Product Overview
Recombinant EBV p18 protein fused to a GST-Tag at C-terminus was expressed in E. coli and purified by proprietary chromatographic technique.
> 95% pure as determined by 10% PAGE (coomassie staining).
50mM Tris-HCl pH 8.0, 60mM NaCl and 50% glycerol.
2-8°C short term, -20°C long term
The Epstein-Barr virus (EBV), also called Human herpes virus 4 (HHV-4), is a virusof the herpes family(which includes Herpes simplex virusand Cytomegalovirus. On infecting the B-lymphocyte, the linear virus genome circularizes and the virus subsequently persists within the cell as an episome. The virus can execute several distinct programs of gene expressionwhich can be broadly categorized as being lytic cycle or latent cycle. The lytic cycleor productive infection results in staged expression of a host of viral proteinswith the ultimate objective of producing infectious virions. Formally, this phase of infection does not inevitably lead to lysis of the host cellas EBV virions are produced by budding from the infected cell. The latent cycle(lysogenic) programs are those that do not result in production of virions. A very limited, distinct set of viral proteins are produced during latent cycle infection. These include Epstein-Barr nuclear antigen(EBNA)-1, EBNA-2, EBNA-3A, EBNA-3B, EBNA-3C, EBNA-leader protein (EBNA-LP) and latent membrane proteins(LMP)-1, LMP-2A and LMP-2B and the Epstein-Barr encoded RNAs(EBERs).
Epstein–Barr virus; Herpesviridae; Gammaherpesvirinae; Lymphocryptovirus; Human herpesvirus 4; HHV-4; EBV; p18 protein; Epstein-Barr Virus (EBV) p18 (VP26); Recombinant EBV (HHV-4) p18 virus capsid antigen (VP26, BFRF3); VP26; BFRF3; EBV p18; Epstein–Barr


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The association between enteric viruses and necrotizing enterocolitis


Authors: Cheng, Chen; He, Yu; Xiao, Sa; Ai, Qing; Yu, Jialin

Studies on necrotizing enterocolitis (NEC) have not largely focused on enteric viruses. In order to demonstrate the association between enteric viruses and NEC, stool specimens of 51 neonates with NEC and 39 "normal" neonates were collected to detect rotavirus (RV), astrovirus (ASV), sapovirus, enterovirus (EV), adenovirus (ADV), Epstein-Barr virus (EBV), cytomegalovirus (CMV), and human bocavirus (HBoV). Rotavirus A (RVA), ASV, EBV, and ADV were detected in both the NEC and control groups; however, EV and HBoV were detected only in the NEC group and CMV was not detected in either group. ASV was the most common enteric virus, but no significant differences were found between NEC and control groups, as was similarly the case for EBV and EV. The prevalence of ADV and HBoV was higher in the NEC group than in the control group (P= 0.011,P= 0.005, respectively) but RVA showed the opposite trend (P= 0.014). Virus positivity or negativity had no influence on the clinical manifestation of NEC. Conclusion: The roles of different viruses in NEC are not congruent. Some, such as ASV, may be regarded as commensal in neonates, while in NEC patients, the presence of ADV and EBV may be related to severity of disease.What is known:center dot The etiology of NEC remains unknown. Studies on necrotizing enterocolitis (NEC) have not largely focused on enteric viruses and the conclusions were inconsistent.What is new:center dot Enteric viruses are common in the gut of neonates, but not all of them are pathogenic.center dot The existence of ADV and EBV may be related to the severity of NEC.

Oral Epstein Barr virus-positive mucocutaneous ulcer: gingival presentation of a benign lymphoproliferative lesion


Authors: Li, D. T. S.; Lo, A. W., I; Su, Y-X

Epstein-Barr virus-positive mucocutaneous ulcer (EBVMCU) is a benign lymphoproliferative lesion related to iatrogenic or age-related immunosuppression in patients with prior Epstein-Barr virus (EBV) infection. Although the clinical presentation may resemble malignant disease, the course of EBVMCU is indolent, and regression is expected when immunosuppression is reduced. We present a case of EBVMCU in the gingiva of a 59-year-old male patient with long-standing pemphigus vulgaris. The initial presentation was suspicious for oral cavity cancer, which was ruled out by biopsy. After reduction of immunosuppression, the ulceration regressed and an area of exposed necrotic bone remained. Complete healing was achieved after sequestrectomy and primary closure with a local gingival flap.

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