Intended Use
This ELISA kit can be applied to the in vitro quantitative determination of 25-hydroxy Vitamin D3 concentrations in serum, plasma and other biological fluids. This kit is for research use only and not to be used in diagnostic procedures.
Contents of Kit
Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution. The volume of reagents in partial shipments is a little more than the volume marked on the label, please use inmeasuring instead of directly pouring.
1. Micro ELISA Plate (Dismountable), 8 wells ×12 strips, -20°C, 6 months
2. Reference Standard, 2 vials, -20°C, 6 months
3. Concentrated Biotinylated Detection Ab (100×), 1 vial, 120 μL, -20°C, 6 months
4. Concentrated HRP Conjugate (100×), 1 vial, 120 μL, -20°C(shading light), 6 months
5. Reference Standard & Sample Diluent, 1 vial, 20 mL, 4°C, 6 months
6. Biotinylated Detection Ab Diluent, 1 vial, 14 mL, 4°C, 6 months
7. HRP Conjugate Diluent, 1 vial, 14 mL, 4°C, 6 months
8. Concentrated Wash Buffer (25×), 1 vial, 30 mL, 4°C, 6 months
9. Substrate Reagent, 1 vial, 10 mL, 4°C (shading light)
10. Stop Solution, 1 vial, 10 mL, 4°C
11. Plate Sealer, 5 pieces
12. Product Description, 1 copy
13. Certificate of Analysis, 1 copy
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 25-hydroxy Vitamin D3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 25-hydroxy Vitamin D3 were tested on 3 different plates, 20 replicates in each plate.
COA
Publication 
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