Human 25 Hydroxy Vitamin D3 ELISA kit (DEIA-BJ648)

Regulatory status: For research use only, not for use in diagnostic procedures.

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serum, plasma, cell culture supernatants, body fluid, tissue homogenate
Species Reactivity
Intended Use
This CD 25-OH VD3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human 25-OH VD3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Contents of Kit
2. ENZYME CONJUGATE: 10 mL, 1 vial
3. STANDARD A: 0 nmol/L, 1 vial
4. STANDARD B: 1.0 nmol/L, 1 vial
5. STANDARD C: 2.5 nmol/L, 1 vial
6. STANDARD D: 5.0 nmol/L, 1 vial
7. STANDARD E: 10 nmol/L, 1 vial
8. STANDARD F: 25 nmol/L, 1 vial
9. SUBSTRATE A: 6 mL, 1 vial
10. SUBSTRATE B: 6 mL, 1 vial
11. STOP SOLUTION: 6 mL, 1 vial
12. WASH SOLUTION (100 x): 10 mL, 1 vial
13. BALANCE SOLUTION: 6 mL, 1 vial
All reagents provided are stored at 2-8°C. Refer to the expiration date on the label.
Intra-assay Precision: 4.4%-5.6%
Inter-assay Precision: 6.6%-7.9%
The sensitivity in this assay is 0.1 nmol/L.


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Null association between serum 25-hydroxyvitamin D levels with allergic rhinitis, allergic sensitization and non-allergic rhinitis: A Mendelian randomization study


Authors: Feng, Qi; Bonnelykke, Klaus; Ek, Weronica E.; Chawes, Bo L.; Yuan, Shuai; Cheung, Ching Lung; Li, Gloria H. Y.; Leung, Raymond Y. H.; Cheung, Bernard M. Y.

Background Previous observational studies have not found a conclusive association between serum 25-hydroxyvitamin D (25(OH)D) levels and allergic rhinitis (AR) or allergic sensitization (AS). Objective To investigate a causal association between 25(OH)D levels with risk of AR and AS, using a two-sample Mendelian randomization (MR) approach. Methods Seven single nucleotide polymorphisms (SNPs), previously shown to be associated with serum 25(OH)D levels, were identified as instrumental variables. The primary outcome was AR, and the secondary outcomes were AS and non-allergic rhinitis (NAR). The genome-wide association (GWA) summary statistics of the outcomes were obtained from two cohort studies (EAGLE Consortium and UK Biobank). An MR analysis with random-effects inverse-variance weighted method was performed as the primary analysis to estimate overall effect size (odds ratio [OR] and 95% confidence interval [CI]). Sensitivity analysis using weighted median method and MR-Egger regression method was conducted. A subgroup analysis based on 25(OH)D synthesis-related SNPs was further applied. Results Serum 25(OH)D levels were not causally associated with risk of AR (OR: 0.960; 95% CI: 0.779-1.184), AS (OR: 1.059; 95% CI: 0.686 to 1.634) or NAR (OR: 0.937; 95% CI: 0.588-1.491). Subgroup analysis also showed null association between 25(OH)D synthesis-related SNPs and the outcomes. Sensitivity analyses yielded similar results. Conclusions and Clinical Relevance This MR study found no evidence supporting a causal association between serum 25(OH)D levels and risk of AR, AS and NAR in European-ancestry population. This argues against the previous postulation that vitamin D supplementation is effective in prevention of allergic diseases.

VITamin D and OmegA-3 TriaL (VITAL): Effects of Vitamin D Supplements on Risk of Falls in the US Population


Authors: LeBoff, Meryl S.; Murata, Elle M.; Cook, Nancy R.; Cawthon, Peggy; Chou, Sharon H.; Kotler, Gregory; Bubes, Vadim; Buring, Julie E.; Manson, JoAnn E.

Context: It is unclear whether vitamin D supplementation reduces risk of falls, and results from randomized controlled trials (RCTs) are conflicting. Objective: The objective of this work is to determine whether 2000 IU/day of supplemental vitamin D 3 decreases fall risk. Design: VITamin D and OmegA-3 TriaL (VITAL) is a double-blind, placebo-controlled RCT including 25 871 adults, randomly assigned November 2011 to March 2014 and treated for 5.3 years (median). Setting: This is a nationwide study. Participants: Men 50 years or older and women 55 years or older (mean age, 67.1 years) without cancer or cardiovascular disease at baseline participated in this study. Interventions: Interventions included vitamin D-3 (cholecalciferol; 2000 IU/day) and/or omega-3 fatty acids (1 g/day) or respective placebos in a 2 x 2 factorial design. Main Outcome Measures: Main outcome measures include 2 or more falls and falls resulting in a doctor or hospital visit. Results: Baseline serum total 25-hydroxyvitamin D (25[OH]D) level was 77 nmol/L; characteristics were well-balanced between groups. Numbers of participants with 2 or more falls were similar between active and placebo groups (9.8% vs 9.4%). Over 5 years, there were no differences in the proportion having 2 or more falls (odds ratio [OR] = 0.97; 95% CI, 0.90-1.05, P=.50), falls resulting in a doctor visit (OR = 1.03; 95% CI, 0.94-1.13, P=.46), or resulting in a hospital visit (OR = 1.04; 95% CI, 0.90-1.19, P=.61) between groups. Results did not differ between those with baseline 25(OH)D less than 50 vs 50 nmol/L or greater or other cut points. Conclusion: Daily supplemental vitamin D-3 vs placebo did not decrease fall risk in generally healthy adults not selected for vitamin D insufficiency. This large RCT does not indicate that supplemental vitamin D should be used for primary prevention of falls in the US population.

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