Native IBV (B/Victoria 504/00) Antigen

Name: Native IBV (B/Victoria 504/00) Antigen
SKU: DAG1619
Rating: 5 (1 reviews)

IBV (B/Victoria 504/00) antigen, purified from embryonated eggs

COA

SDS

Datasheet

Summary

Product Overview

Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza B virus, strain B/Victoria/504/00. The Influenza B Virus was purified by Ultracentrifugation with 10-40 % sucrose gradient.

Nature

Native

Purity

≥90% as determined by SDS-PAGE

Format

Liquid

Buffer

20mM PB, 150mM NaCl, pH7.4

Preservative

None

Storage

Store at -20°C to -80°C. Avoid multiple freeze/thaw cycles.

Introduction

The Influenza B virus is an enveloped member of the Orthomyxoviridae family. Influenza B, along with Influenza A, is responsible for the respiratory illness influenza (flu). Influenza B differs from influenza A in that influenza B only infects humans and seals, while influenza A infects a wide range of animals, including birds and pigs. This increased host range allows influenza A to mutate about 2-3 times faster than influenza B.

Keywords

Influenza B virus; IBV

Inactivation

β-propiolactone (BPL)

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

Influenza Virus Antigens

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Customer Reviews

References

Molecular characteristics and pathogenicity analysis of QX-like avian infectious bronchitis virus isolated in China in 2017 and 2018

POULTRY SCIENCE

Authors: Yan, Shihong; Sun, Yali; Huang, Xiuying; Jia, Wenfeng; Xie, Deqiong; Zhang, Guozhong

Abstract

Proportions of QX-like genotype infectious bronchitis virus (IBV) isolates have increased over time. Here, to better understand the epidemiology and pathogenicity of IBV in China and control the spread of infectious bronchitis (IB), we conducted sequence analyses and examined the pathogenicity of 5 field isolates from diseased flocks in 2017 and 2018. Sequence analyses revealed that all the 5 strains, as well as many recent field isolates from other researchers, belonged to the QX-like IBV genotype, which were distantly related to commercial vaccine strains. Viral pathogenicity experiments showed that the isolates caused high morbidity and severe ciliostasis in chickens, although they caused milder lethality. This provides further evidence that QX-like IBV emergence remains a major problem in the poultry industry, and information on IBV epidemiology and pathogenicity may help to control IB.

Multiple novel non-canonically transcribed sub-genomic mRNAs produced by avian coronavirus infectious bronchitis virus

JOURNAL OF GENERAL VIROLOGY

Authors: Keep, Sarah; Oade, Michael S.; Lidzbarski-Silvestre, Filip; Bentley, Kirsten; Stevenson-Leggett, Phoebe; Freimanis, Graham L.; Tennakoon, Chandana; Sanderson, Nicholas; Hammond, John A.; Jones, Richard C.; Britton, Paul; Bickerton, Erica

Abstract

Coronavirus sub-genomic mRNA (sgmRNA) synthesis occurs via a process of discontinuous transcription involving complementary transcription regulatory sequences (TRSs), one (TRS-L) encompassing the leader sequence of the 5' untranslated region (UTR), and the other upstream of each structural and accessory gene (TRS-B). Several coronaviruses have an ORF located between the N gene and the 3'-UTR, an area previously thought to be non-coding in the Gammacoronavirus infectious bronchitis virus (IBV) due to a lack of a canonical TRS-B. Here, we identify a non-canonical TRS-B allowing for a novel sgmRNA relating to this ORF to be produced in several strains of IBV: Beaudette, CR88, H120, D1466, Italy-02 and QX. Interestingly, the potential protein produced by this ORF is prematurely truncated in the Beaudette strain. A single nucleotide deletion was made in the Beaudette strain allowing for the generation of a recombinant IBV (rIBV) that had the potential to express a full-length protein. Assessment of this rIBV in vitro demonstrated that restoration of the full-length potential protein had no effect on viral replication. Further assessment of the Beaudette-derived RNA identified a second non-canonically transcribed sgmRNA located within gene 2. Deep sequencing analysis of allantoic fluid from Beaudette-infected embryonated eggs confirmed the presence of both the newly identified non-canonically transcribed sgmRNAs and highlighted the potential for further yet unidentified sgmRNAs. This HiSeq data, alongside the confirmation of non-canonically transcribed sgmRNAs, indicates the potential of the coronavirus genome to encode a larger repertoire of genes than has currently been identified.