Human Influenza B IgA ELISA Kit (DEIA2371)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma
Species Reactivity
Human
Intended Use
This product has been designed for the the detection and the quantitative determination of specific IgA antibodies against Influenza B in serum and plasma.
Contents of Kit
1. Microtiter Strips
2. Calibrator A (Negative Control)
3. Calibrator B (Cut-Off Standard)
4. Calibrator C (Weak Positive Control)
5. Calibrator D (Positive Control)
6. Enzyme Conjugate 15 mL, anti-human-IgA-HRP (rabbit), in protein-containing buffer solution. Addition of 0.01 % methylisothiazolone and 0.01 % bromonitrodioxane and 5 mg/L ProclinTM.
7. Substrate 15 mL, TMB (tetramethylbenzidine).
8. Stop Solution 15 mL, 0.5 M sulfuric acid.
9. Sample Diluent 60 mL, PBS/BSA buffer. Addition of 0.095 % sodium azide.
10. Washing Buffer 60 mL, PBS + Tween 20, 10X concentrate.
Storage
Store at 2-8°C. Do not use past kit expiration date. For more detailed information, please download the following document on our website.
Sensitivity
Clinical Sensitivity: 85%
Analytical Sensitivity: 1.38 U/mL

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References


The challenges of teaching strategic management: Including the institution based view

INTERNATIONAL JOURNAL OF MANAGEMENT EDUCATION

Authors: Napshin, Stuart A.; Marchisio, Gaia

Dealing with the institutional differences is increasingly important in the globalizing world economy. While an Institutional Based View (IBV) is gaining prominence in strategic management scholarship, it is under-represented in strategic management courses. The resource based view (RBV) and Industrial Organization Economics (IO) commonly represented by Porter's Five Forces, remain the significant theories within strategic management courses in the U.S. We summarize the tenants of the Institutional Based View, demonstrate how it relates to the Resource Based View and Industrial Organization Economics and present an experiential exercise for introducing basic aspects of the IBV in the strategic management classroom. (C) 2017 Elsevier Ltd. All rights reserved.

Evolutionary Analysis of Infectious Bronchitis Virus Reveals Marked Genetic Diversity and Recombination Events

GENES

Authors: Rohaim, Mohammed A.; El Naggar, Rania F.; Abdelsabour, Mohammed A.; Mohamed, Mahmoud H. A.; El-Sabagh, Ibrahim M.; Munir, Muhammad

In the last 5 years, frequent outbreaks of infectious bronchitis virus (IBV) are observed in both broiler and layer chicken flocks in the Kingdom of Saudi Arabia (KSA) in spite of extensive usage of vaccines. The IBV is a widespread avian coronavirus affecting both vaccinated and unvaccinated chicken flocks and is attributed to significant economic losses, around the globe. In the present study, 58 (n= 58) samples were collected from four different commercial poultry flocks from 8 KSA districts during 2019. A total of nine positive isolates (9/58; 15.5%), based on real-time reverse transcriptase PCR targeting nucleocapsid (N) gene, were used for further genetic characterization and evolutionary analysis. Genetic characterization of the partial spike (S1) gene revealed the clustering of the reported isolates into three different genotypes, whereas four additional isolates were grouped within 4/91 genotype, two isolates within IS/885 genotype, one isolate was closely related to IS/1494/06, and two isolates were grouped within classic serotype (vaccine-like strains). Phylodynamic revealed clustering of four isolated viruses within GI-13 lineage, three isolates within GI-23 lineage, and two isolates within GI-1 lineage. Results indicate that there are high evolutionary distances between the newly identified IBV strains in this study and the commercially used vaccines (GI-1), suggesting that IBV strains circulating in the KSA are under constant evolutionary pressures. Selective pressure biostatistics analyses consistently demonstrate the presence of a higher positive score which highlights the role of natural selection, a mechanism of virus evolution on sites located on the protein surface, within or nearby domains involved in viral attachment or related functions. Recombination analysis revealed emergence of two isolates through recombination events resulting in new recombinant viruses. Taken together, these finding demonstrate the genetic and evolutionary insights into the currently circulating IBV genotypes in KSA, which could help to better understand the origin, spread, and evolution of infectious bronchitis viruses, and to ascertain the importance of disease monitoring as well as re-evaluation for the currently used vaccines and vaccination programs.

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