Native IBV (B/Florida 04/06) Antigen

Name: Native IBV (B/Florida 04/06) Antigen
SKU: DAG1622
Rating: 5 (1 reviews)

Native IBV (B/Florida 04/06) Antigen from embryonated eggs

COA

SDS

Datasheet

Summary

Product Overview

Allantoic fluid of 10 days old embryonated eggs, inoculated with influenza B virus, strain B/Florida/04/06. The Influenza B Virus was purified by Ultracentrifugation with 10-40 % sucrose gradient.

Nature

Native

Tag/Conjugate

Unconjugated

Alternative Names

Influenzavirus B; Influenza B virus; IBV

Purity

Greater than 90.0% as determined by SDS-PAGE.

Format

Liquid

Buffer

STE, 0.1 % sodium azide (NaN3) and 0.005 % thimerosal.

Preservative

0.1 % Sodium Azide, 0.005 % thimerosal

Storage

2-8°C short term, -20°C long term

Introduction

The Influenza B virus is an enveloped member of the Orthomyxoviridae family. Influenza B, along with Influenza A, is responsible for the respiratory illness influenza (flu). Influenza B differs from influenza A in that influenza B only infects humans and seals, while influenza A infects a wide range of animals, including birds and pigs. This increased host range allows influenza A to mutate about 2-3 times faster than influenza B.

Keywords

Influenzavirus B; Influenza B virus; IBV

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Documents

pdf COA

pdf SDS

pdf Datasheet

Influenza Virus Antigens

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References

Emergence of variant avian infectious bronchitis virus in India

IRANIAN JOURNAL OF VETERINARY RESEARCH

Authors: Raja, A.; Raj, Dhinakar G.; Kumanan, K.

Abstract

Background: Infectious bronchitis virus (IBV) is the etiological agent of an acute and highly contagious disease. Infectious bronchitis (IB) affects chicken of all ages and poses major economic loses to the poultry industry worldwide. The continuous evolution of the spike protein (S1) of IBV is responsible for the prevalence of many serotypes/genotypes around the world. Multiple lineages of IBV strains have been detected in chicken flocks in India since 2003. Aims: To detect IBV genotypes prevalent in India. Methods: Organ samples from 20 IBV-positive flocks with variable clinical signs were used for the amplification of the S1 gene of IBV by reverse transcriptase-polymerase chain reaction (RT-PCR). Results: Positive PCR amplicons were sequenced. Sequence analysis showed that 14 field isolates belonged to the GI-1 genetic lineage (Mass 41 serotype), two field isolates belonged to the GI-13 (UK 4/91 variant IBV strain), one field isolate grouped with GIB, GV, and GVI genetic lineage and three belonged to a variant genotype unique to India (GI-24). Phylogenetic analysis also showed a similar type of grouping within the field isolates. Among the fourteen GI-1 isolates, 12 were isolated between 2003 and 2006 and only two were isolated between 2009 and 2011. The two field isolates belonging to GI-13 were isolated in 2007, another one belonging to GIB, GV, and GVI was isolated in 2010 and three field isolates were not close to any reference IBV sequences isolated in 2006 (IND-TN-168-06), 2010 (IND-TN-280-10) and 2011 (IND-TN-290-11). Conclusion: A unique variant of IBV is emerging in India (GI-24). Our findings will have important implications for future vaccine intervention.

Infectious Bronchitis Virus Infection Induces Apoptosis during Replication in Chicken Macrophage HD11 Cells

VIRUSES-BASEL

Authors: Han, Xiaoxiao; Tian, Yiming; Guan, Ru; Gao, Wenqian; Yang, Xin; Zhou, Long; Wang, Hongning

Abstract

Avian infectious bronchitis has caused huge economic losses in the poultry industry. Previous studies have reported that infectious bronchitis virus (IBV) infection can produce cytopathic effects (CPE) and apoptosis in some mammalian cells and primary cells. However, there is little research on IBV-induced immune cell apoptosis. In this study, chicken macrophage HD11 cells were established as a cellular model that is permissive to IBV infection. Then, IBV-induced apoptosis was observed through a cell viability assay, morphological changes, and flow cytometry. The activity of caspases, the inhibitory efficacy of caspase-inhibitors and the expression of apoptotic genes further suggested the activation of apoptosis through both intrinsic and extrinsic pathways in IBV-infected HD11 cells. Additionally, ammonium chloride (NH4Cl) pretreated HD11 cells blocked IBV from entering cells and inhibited IBV-induced apoptosis. UV-inactivated IBV also lost the ability of apoptosis induction. IBV replication was increased by blocking caspase activation. This study presents a chicken macrophage cell line that will enable further analysis of IBV infection and offers novel insights into the mechanisms of IBV-induced apoptosis in immune cells.