Chicken Infectious Bronchitis Virus IgY ELISA Kit (DElABL40)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
Serum, Plasma, other biological fluids
Species Reactivity
Chick
Intended Use
The Chicken Infectious Bronchitis Virus IgY (CIB-IgY) ELISA Kit is to be used for the vitro quantitative determination of Chicken CIB-IgY in Serum, Plasma and other biological fluids. The Kit is intended for research use only, not for diagnostic or therapeutic procedure. If detection of other special sample, please contact our technical support.
Contents of Kit
1. Wash solution: 20 mL×1bottle
2. HRP-Conjugate reagent: 6 mL×1 bottle
3. Microelisa stripplate: 12well×8strips
4. Sample diluent: 6 mL×1 bottle
5. Chromogen Solution A: 6 mL×1 bottle
6. Chromogen Solution B: 6 mL×1 bottle
7. Standard 200ng/ml: 0.5 mL×1 bottle
8. Standard diluent: 6 mL×1 bottle
9. Stop Solution: 6 mL×1 bottle
10. Instruction: 1
11. Closure plate membrane: 2
12. Sealed bags: 1
Storage
Storage: 2-8°C
Validity: 6 months
Precision
Intra-Assay: CV≤ 15%
Inter-Assay: CV≤15%
Detection Range
6.8ng/mL - 220ng/mL
Sensitivity
< 2.0ng/mL

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References


ANP32 Proteins Are Essential for Influenza Virus Replication in Human Cells

JOURNAL OF VIROLOGY

Authors: Staller, Ecco; Sheppard, Carol M.; Neasham, Peter J.; Mistry, Bhakti; Peacock, Thomas P.; Goldhill, Daniel H.; Long, Jason S.; Barclay, Wendy S.

ANP32 proteins have been implicated in supporting influenza virus replication, but most of the work to date has focused on the ability of avian Anp32 proteins to overcome restriction of avian influenza polymerases in human cells. Using a CRISPR approach, we show that the human acidic nuclear phosphoproteins (ANPs) ANP32A and ANP32B are functionally redundant but essential host factors for mammalian-adapted influenza A virus (IAV) and influenza B virus (IBV) replication in human cells. When both proteins are absent from human cells, influenza polymerases are unable to replicate the viral genome, and infectious virus cannot propagate. Provision of exogenous ANP32A or ANP32B recovers polymerase activity and virus growth. We demonstrate that this redundancy is absent in the murine Anp32 orthologues; murine Anp32A is incapable of recovering IAV polymerase activity, while murine Anp32B can do so. Intriguingly, IBV polymerase is able to use murine Anp32A. We show, using a domain swap and point mutations, that the leucine-rich repeat (LRR) 5 region comprises an important functional domain for mammalian ANP32 proteins. Our approach has identified a pair of essential host factors for influenza virus replication and can be harnessed to inform future interventions. IMPORTANCE Influenza virus is the etiological agent behind some of the most devastating infectious disease pandemics to date, and influenza outbreaks still pose a major threat to public health. Influenza virus polymerase, the molecule that copies the viral RNA genome, hijacks cellular proteins to support its replication. Current anti-influenza drugs are aimed against viral proteins, including the polymerase, but RNA viruses like influenza tend to become resistant to such drugs very rapidly. An alternative strategy is to design therapeutics that target the host proteins that are necessary for virus propagation. Here, we show that the human proteins ANP32A and ANP32B are essential for influenza A and B virus replication, such that in their absence cells become impervious to the virus. We map the proviral activity of ANP32 proteins to one region in particular, which could inform future intervention.

Progress on chicken T cell immunity to viruses

CELLULAR AND MOLECULAR LIFE SCIENCES

Authors: Dai, Manman; Xu, Chenggang; Chen, Weisan; Liao, Ming

Avian virus infection remains one of the most important threats to the poultry industry. Pathogens such as avian influenza virus (AIV), avian infectious bronchitis virus (IBV), and infectious bursal disease virus (IBDV) are normally controlled by antibodies specific for surface proteins and cellular immune responses. However, standard vaccines aimed at inducing neutralizing antibodies must be administered annually and can be rendered ineffective because immune-selective pressure results in the continuous mutation of viral surface proteins of different strains circulating from year to year. Chicken T cells have been shown to play a crucial role in fighting virus infection, offering lasting and cross-strain protection, and offer the potential for developing universal vaccines. This review provides an overview of our current knowledge of chicken T cell immunity to viruses. More importantly, we point out the limitations and barriers of current research and a potential direction for future studies.

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