Anti-FTO (aa 50-150) polyclonal antibody

In this work, a new sensitive enzyme-based electrode for electrochemical cholesterol biosensor was fabricated based on a nanocomposite of Au nanoparticles, ZnO nanoparticles and multi-wall carbon nanotubes (Au/ZnO/MWCNTs). The nanocomposite was prepared by sol-gel method and deposited on FTO substrate by dip coating, followed by cholesterol oxidase (ChOx) enzyme immobilized (ChOx/Au/ZnO/MWCNTs). Structural properties and morphology of the nanocomposite have been studied using X-ray diffraction (XRD) and Field emission scanning electron microscopy (FESEM). The sample was subjected to Fourier transform infrared spectroscopy (FTIR) to determine functional groups. Electrochemical behavior of the electrode was studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques as a function of cholesterol concentration. Electrochemical impedance spectroscopy (EIS) was also considered to study of surface modified electrodes. The ChOx/Au/ZnO/MWCNTs electrode has been found to have enhanced electron transfer and display excellent analytical linear performances. The fabricated electrode exhibited low detection limit (0.1 mu M), high sensitivity (25.89 mu A/mu M) evaluated from DPV data in the detection range of 0.1-100 mu M and high selectivity in the determination of cholesterol over glucose and uric acid. The application of the ChOx/Au/ZnO/MWCNTs electrode in detection of cholesterol in human serum was also confirmed.

In the present investigation, a simple sol-gel approach has been employed for the synthesis of hexagonal wurtzite ZnO nanoparticles (NPs). X-ray diffraction (XRD) rietveld study and Raman analysis reveal the mislaid of the mixed phases of ZnO NPs after doping. The scanning electron micrographs and transmission electron microscopy confirm the formation of hexagonal disk-shaped NPs. FTIR, PL, EDX, XPS validate optical and stoichiometric concentration of Co in ZnO lattice. UV-visible DRS study shows absorption edge with humps to the red region of the spectrum with increasing the absorption strength and shortening of band gap as the content of Co (II) increases. Finally, the prepared samples were coated on the surface of cleaned FTO substrate through a simple doctor blade technique and sensitized with prepared dye. The ZnO photoanode shows 0.036% of efficiency (eta%) while the photoanodes of Co doped samples expose the enhanced efficiencies of 0.29%, 0.389%, 0.089%.