Sample
serum, plasma, cell culture supernatants, tissue homogenate
Detection Method
Sandwich ELISA
Intended Use
For quantitative detection of Rat Hspa8 (Heat shock cognate 71 kDa protein) in serum, plasma, tissue homogenates and other biological fluids.
Contents of Kit
1. 96-well strip plate (Dismountable), 1 plate
2. Lyophilized Standard, 2 vials
3. Sample/Standard dilution buffer, 20 mL
4. Biotin-detection antibody (Concentrated), 120 uL
5. Antibody dilution buffer, 10 mL
6. HRP-Streptavidin Conjugate(SABC), 120 uL
7. SABC dilution buffer, 10 mL
8. TMB substrate, 10 mL
9. Stop solution, 10 mL
10. Wash buffer (25X), 30 mL
11. Plate Sealer, 5 pieces
12. Product Manual, 1 copy
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Detection Range
0.625-40 ng/mL
Citations
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Q & A
Q: May I ask if the antibodies used for these kits are polyclonal or monoclonal?
A: Mouse monoclonal antibodies.
Customer Reviews
Elliott, RMA; Lloyd, RE; et al. Effects of HSPA8, an evolutionarily conserved oviductal protein, on boar and bull spermatozoa. REPRODUCTION 137:191-203(2009).
Son, YS; Park, JH; et al. Heat shock 70-kDa protein 8 isoform 1 is expressed on the surface of human embryonic stem cells and downregulated upon differentiation. STEM CELLS 23:1502-1513(2005).
COA
Certificate of Analysis
