Human HSPA8 (Heat shock cognate 71 kDa protein) ELISA Kit

Sample

Cell lysates, tissue homogenates and other biological fluids

Species Reactivity

Human

Detection Method

sELISA

Intended Use

For quantitative detection of HSPA8 in cell lysates, tissue homogenates and other biological fluids.

Contents of Kit

1. ELISA Microplate(Dismountable), 8×12, 2-8°C/-20°C
2. Lyophilized Standard, 2vial, 2-8°C/-20°C
3. Sample Dilution Buffer, 20ml, 2-8°C
4. Biotin-labeled Antibody(Concentrated), 120ul, 2-8°C(Avoid Direct Light)
5. Antibody Dilution Buffer, 10ml, 2-8°C
6. HRP-Streptavidin Conjugate(SABC), 120ul, 2-8°C(Avoid Direct Light)
7. SABC Dilution Buffer, 10ml, 2-8°C
8. TMB Substrate, 10ml, 2-8°C(Avoid Direct Light)
9. Stop Solution, 10ml, 2-8°C
10. Wash Buffer(25×), 30ml, 2-8°C
11. Plate Sealer, 5pieces
12. Product Description, 1copy

Storage

2-8°C

Performance Characteristics

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% within the expiration date under appropriate storage condition.

To minimize extra influence on performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is strongly suggested that the same operator performs the whole assay from the beginning to the end.

Precision

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Detection Range

0.313-20ng/ml

Sensitivity

0.188ng/ml

Standard Curve

Results of a typical standard operation of a HSPA8 ELISA Kit are listed below. This standard curve was generated at our lab for demonstration purpose only. Users shall obtain standard curve as per experiment by themselves. (N/A=not applicable)