Pseudotyped VSV-SARS-CoV-2 S-ΔG-Luciferase (Omicron variant, XBB)

Product Overview

Pseudotyped VSV-SARS-CoV-2 S-ΔG-Luciferase (Omicron variant, XBB) encodes the antigenomic-sense (or positive-sense) RNA of a replicaton-restricted recombinant vesicular stomatitis virus (rVSV) in which the glycoprotein (G) gene has been replaced with SARS-CoV-2 spike protein (T19I, Δ24-26, A27S, V83A, G142D, Δ144, H146Q, Q183E, V213E, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460S, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K). Because the infectivity of Pseudotyped VSV-SARS-CoV-2 S-ΔG-Luciferase is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices.The pseudotype VSV particles encode Luciferase together with the VSV nucleocapsid (N), phosphoprotein (P), glycoprotein (G), and large polymerase subunit (L) in their pVSV-ΔG vector. When the VSV pseudovirus infect the target cells, Luciferase expression is proportional to the number of cells that were infected.

Nature

Virus

Alternative Names

SARS-CoV-2; Coronavirus; SARS-CoV; SARS-CoV-2 Spike Protein; Severe Acute Respiratory Syndrome; Severe Acute Respiratory Syndrome-1; Severe Acute Respiratory Syndrome glycoprotein; Severe Acute Respiratory Syndrome-1 glycoprotein; SARS-CoV-2 Pseudovirus; SARS-CoV Pseudovirus; SARS Pseudovirus; SARS-CoV-2 RVP; SARS-CoV-2 Reporter Virus Particle; VSV pseudovirus

Storage

Store at -80°C. Multiple freeze/thaw cycles not recommended.
When using the virus, transfer the virus from the -80° C refrigerator and melt it in an ice bath.

Ship

Frozen on dry ice