Product Overview
Pseudotyped LV-SARS-CoV-2 S-ΔG-Luciferase+GFP (D614G) encodes the antigenomic-sense (or positive-sense) RNA of a replicaton-restricted recombinant vesicular stomatitis virus (rVSV) in which the glycoprotein (G) gene has been replaced with SARS-CoV-2 spike protein. Because the infectivity of Pseudotyped LV-SARS-CoV-2 S-ΔG-Luciferase+GFP (D614G) is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices.The pseudotype VSV particles encode Luciferase and GFP together with the VSV nucleocapsid (N), phosphoprotein (P), glycoprotein (G), and large polymerase subunit (L) in their pVSV-ΔG vector. When the VSV pseudovirus infect the target cells, Luciferase expression is proportional to the number of cells that were infected.
Alternative Names
SARS; SARS-CoV; SARS-CoV-2; Pseudotyped SARS-CoV-2; D614G
Application Notes
We recommended to use 2-5 uL pseudotyped virus per 1E+04 293T cells for in vitro assay. Due to differences in cell status,the best infection conditions and MOI should be determined by the end user. The virus can be diluted with cell culture medium if needed.
Storage
Store at -80°C. Multiple freeze/thaw cycles not recommended. When using the virus, transfer the virus from the -80° C refrigerator and melt it in an ice bath.
Antigen Description
SARS-CoV-2
Keywords
SARS;SARS-CoV;SARS-CoV-2;Pseudotyped SARS-CoV-2;D614G
Synonyms
SARS; SARS-CoV; SARS-CoV-2; Pseudotyped SARS-CoV-2; D614G
Citations
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