Anti-Salmonella type E Monoclonal antibody, Clone 54-89

Gold nanoparticles capped with chitosan (CH-NGs), glycol chitosan (GC-NGs) and poly(gamma-glutamic acid) (PANGs) were synthesized separately, characterized and evaluated for catalytic and antibacterial activities. Surface Plasmon resonance peak at 520-530 nm confirmed the formation of NGs, while FTIR spectra revealed the involvement of hydroxyl, amine and amide groups in biopolymers on NGs formation and coating. Particle size, zeta potential and surface coating were respectively 21.7 nm, +50.2 mV and 20% for CH-NGs, 5.6 nm, +46.5 mV and 43.5% for GC-NGs and 7.4 nm, -37.3 mV and 34.5% for PA-NGs. Compared to citrate-capped NGs (CT-NGs), biopolymer-capped NGs exhibited high catalytic activity in a 4-nitrophenol reduction model with the pseudo first-order catalytic rate for PA-NGs being 4-6 fold higher than CH-NGs and GC-NGs. No significant antibacterial effect was shown for CT-NGs. However, PA-NGs was superior to gentamycin in inhibiting Salmonella enterica and Escherichia coli-O157:H7, while CH-NGs and GC-NGs showed the highest antibacterial effect against Listeria monocytogenes, followed by Salmonella enterica, Escherichia coli-O157:H7, methicillin-resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus. TEM images showed that GC-NGs were attached on MRSA surface to alter cell permeability, block nutrient flow and disrupt cell membrane, whereas PA-NGs penetrated into Salmonella enterica to generate cavities, plasmolysis and disintegration. (c) 2020 Elsevier B.V. All rights reserved.

According to the United States Food and Drug Administration (FDA) agency, bacteriophage solutions targeting the serotype O157:H7 are Generally Recognized as Safe (GRAS) to control STEC during beef processing. However, outbreaks involving the "Big Six" STEC increased the industry concern about those serotypes. The objective of this study was to test the efficacy of MS bacteriophages to reduce the "Big Six" non-O157 STEC in beef. The lysing efficacy of phages isolated for each specific serotype varied from 96.2% to 99.9% in vitro. When applied to contaminated trim, reductions ranging from 0.7 to 1.3 Log of all STEC were observed in ground beef. Bacteriophages may provide an additional barrier against the "Big Six" STEC in ground beef. Results of this research provide support documentation to the FDA to extend GRAS status for bacteriophages as processing aids against all adulterant STEC.