Sheep Serum and Plasma
The Salmonella abortusovis test is an Enzyme-linked immunosorbent assay (ELISA) intended for the detection of IgG anti-Salmonella abortusovis in sheep serum and plasma samples. The test is designed to be used for the diagnosis of abortive salmonellosis infection and evaluation of antibody response to vaccination.
Contents of Kit
Microtiter strips (12 x 8 well strips): 1 microplate coated with S. abortusovis antigen, preserved with sodium azide 0,02%
Buffer A: 50ml ready to use, with preservative
Buffer B (Wash Buffer 10X concentrate):100ml, to dilute to 1X with distilled water; with preservative
HRP-conjugated secondary antibody: 13ml ready to use, with preservative -ABTS Solution: 13ml, ready to use, with preservative
Negative control: lyophilized (prepared in Buffer A)
Positive control: lyophilized (prepared in Buffer A)
Diagnostic sensitivity: 96%
Salmonella enterica spp. enterica serovar abortusovis (Salmonella abortusovis) is a Salmonella serovar host-adapted to sheep, which causes infections that are mainly characterized by abortion as a main symptom. The disease develops in the last weeks of pregnancy, and the pathogenic mechanisms involved have not yet been understood. In the areas endemic for the microorganism, abortion may
occur in up to 50% of the ewes in a flock, usually during the first pregnancy. This high incidence of salmonellosis represents a major threat to the flocks and may result in important economic losses in regions that depend on shepherding.
S. abortusovis is reportable to the World Organization for Animal Health (OIE), but outbreaks are uncommonly described outside a few regions, such as southern Europe and Western Asia. Diagnosis is made by culture of placenta, fetus, or uterine discharge. Isolation of aborting ewes and destruction of contaminated bedding and of all products of abortion reduce contamination. Prevention is
mainly based on vaccination with dead or living vaccines in endemic areas.