Anti-Salmonella Polyclonal antibody

Essential oils in combination with other antimicrobials can be added to food products to reduce the levels of target microbes lower than the infectious dose required to cause human illness. The purpose of this study was to investigate the antimicrobial efficacy of white mustard essential oil (WMEO) and carvacrol against Salmonella in ground chicken stored at 4 and 10 degrees C. At 4 degrees C, 0.75% WMEO10.1% carvacrol treatment had significantly lower (P<0.05) Salmonella at the end of 12-day storage than the control, which contained no antimicrobials. A combination of 0.75% WMEO and 0.01% carvacrol had a bacteriostatic effect against Salmonella in ground chicken samples stored at 10 degrees C for 7 D. The application of the antimicrobials controlled the growth of Salmonella by delaying the exponential phase at temperature abuse and reducing levels of Salmonella to less than the positive control at 4 degrees C. The use of WMEO and carvacrol shows potential in reducing levels of Salmonella under refrigerated conditions and controlling its growth under temperature abuse conditions in raw poultry products. Further research is needed to investigate the toxicity of the compounds and the most efficient way to apply it to a food product to maximize antimicrobial activity.

Salmonella is a major cause of morbidity and mortality in humans worldwide, and the infection with multidrugresistant strains can cause severe diseases. Many coastal cities around the world discharge their wastewaters into the marine environment. These wastewaters contain a variety of pathogenic microorganisms that may have a role in the contamination of this ecosystem and have potential risks for public health. Using an environmental approach, the present study investigated the presence of Salmonella in sediment and water samples collected from Port Blair Bays. In this environmental approach, the provided information about the diversity of the Salmonella serovars, antibiotic resistance and the prevalence of virulence factors in Salmonella, especially from the coastal waters of Port Blair Bays. The occurrence of Salmonellae was significantly higher in water column samples (2.9%) than in those taken from the marine sediments (0.7%). Of the 133 positive Salmonella strains, 22 different serovars were identified. Salmonella enterica serovar Senftenberg was the predominant serovar, being represented by 54 isolates (42.5%), followed by serovar Typhimurium (19 isolates [15%]) and serovar Agona (12 isolates [9.4%]). The presence of virulence genes (filC, sitC, hilA, invA, sipC, hilD, hilC, invF, invE, invH, sipF, aadA, pare, gyrA, spaP and parC) and susceptibility studies with 10 selected antibiotics were also performed. The results of this study revealed that all Salmonella isolates were positive for targeted virulence genes and were resistant to at least one antibiotic. Antibiotic susceptibility studies revealed the presence of multidrug resistant Salmonella strains in coastal water, which usually from land base sources end up in the marine environment and may pose a significant risk on public health.