Anti-Salmonella type H-z24 Polyclonal antibody

A 5' amine group-linked haemagglutinin (HA) gene-specific probe was attached over the surface of a working electrode to develop a rapid, specific, and sensitive point of care detection assay for H1N1 (swine flu) in human respiratory nasal swabs. The probe was attached with a cysteine covered screen-printed gold electrode via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS). The electrochemical assay was performed using differential pulse voltammetry with the use of the redox indicator methylene blue for the detection of different concentrations of the single-stranded viral genome. The developed genosensor showed high sensitivity for H1N1 influenza virus with a detection limit of 0.002 ng/6 mu L of viral nucleic acid in the sample. Samples were analysed by quantitative real-time Polymerase Chain Reaction as well as by conventional PCR. The genosensor showed high specificity, as no cross-reaction was observed with the hetemlogous nucleic acid of different pathogens (Salmonella typhi, Neisseria meningitides, and Streptococcus pyogenes) and human DNA, and it was specific for H1N1 with a sensitivity of similar to 49 mu A cm(-2) ng(-1). Genosensor is based on a very simple methodology that can be followed based on its easy-to-access approach. It is quick and could be used as a point-of-care test for the detection of influenza virus within 30 min.

A novel impedance biosensor was developed based on the interdigitated microelectrodes using H2O2 to reduce SiO2@MnO2 nanocomposites into Mn2+, resulting in significant impedance changes in the rapid and ultrasensitive detection of Salmonella typhimurium (S. typhimurium). The captured S. typhimurium monoclonal antibodies (MAb1) were assembled on the outer layer of the magnetic beads (MBs) for specific enrichment and isolation of S. typhimurium cells in complex samples. The recognized S. typhimurium monoclonal antibodies (MAb(2)) were immobilized on the surface of the SiO2@MnO2 nanocomposites, which could react with the MBs- S. typhimurium conjugates to form the MBs- S. typhimurium -SiO2@MnO2 sandwich complexes. The MnO2 on the surface of the sandwich complexes was reduced into Mn2+ by using H2O2 achieving obvious impedance change that could be detected by the interdigitated microelectrodes. The recoveries for S. typhimurium cells at the concentrations between 2.0 x 10(1) and 2.0 x 10(5) CFU/mL were 83.1 %-97.0 % in the spiked milk samples. The limit of detection of this biosensor for S. typhimurium cells in the spiked milk samples was 21 CFU/mL. This approach possesses the merits of simple operation, high sensitivity, and low cost, potentially enabling this device to be a lab-on-a-chip for rapid screening of foodborne pathogens.