Mouse Anti-Human ITGB2 Hybridoma [UT2/29.2.3.22]

Immunogen

Human cytolytic T lymphocytes

Fusion Species

Mouse X Mouse Hybridoma

Immunological Donor

Mouse spleen

Myeloma

P3X63Ag8.653

Clone

UT2/29.2.3.22

Cell Line Description

Animals were immunized with human cytolytic T lymphocytes. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The beta subunit of LFA-1 is a 95000 dalton polypeptide that is identical to the beta subunits of Mac-1 and the p150, 95 antigens. The antibody will inhibit HLA DR mediated T cell cytotoxicity. The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost. Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells. Tested and found negative for ectromelia virus (mousepox).

Morphology

Lymphoblast

Growth Properties

Suspension

Subculturing

Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml

Storage

Liquid nitrogen vapor phase.

Synonyms

ITGB2; integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); CD18, integrin, beta 2 (antigen CD18 (p95), lymphocyte function associated antigen 1; macrophage antigen 1 (mac 1) beta subunit), MFI7; integrin beta-2; LFA 1; MAC 1; integrin beta chain, beta 2; complement receptor C3 beta-subunit; complement receptor C3 subunit beta; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta; cell surface adhesion glycoprotein LFA-1/CR3/P150, 959 beta subunit precursor); LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1;

Entrez Gene ID

UniProt ID