Human peripheral blood lymphocytes from a patient with high numbers of gamma-delta t cells
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Clone
CBH-96E21
Cell Line Description
Described as secreting a mouse monoclonal antibody (igg1) CD31; the cell line was established in 1988 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with human peripheral blood lymphocytes from a patient with high numbers of gamma-delta T cells confirmed as mouse by Species-PCR. . Viruses: confirmed as mouse by Species-PCR
Morphology
round cells, growing singly and in small clumps
Growth Properties
in small clumps
CultureMethod
Subculturing
Split ratio: 1: 2 , every 3 days; seed out at 0.2 x 10^6 cells/ml; Maintain at 0.2-0.8 x 10^6 cells/ml; Doubling time: 72 hours; Harvest: maximal density of about 0.8 x 10^6 cells/ml.
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