Human Platelet/Endothelial Cell Adhesion Molecule, PECAM1 ELISA Kit

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

The human PECAM1 ELISA kit is for the quantitative determination of human PECAM1.
This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates.

Contents of Kit

1. Bring all reagents to room temperature before use.
2. Capture Antibody: 0.5 mg/mL of mouse anti-PECAM1 monoclonal antibody.
3. Detection Antibody: 0.5 mg/mL rabbit anti-PECAM1 polyclonal antibody conjugated to horseradish-peroxidase(HRP). Dilute to a working concentration of 2.0 μg/mL in detection antibody dilution buffer before use.
4. Standard: Each vial contains 600 ng of recombinant PECAM1. Reconstitute with 1 mL detection antibody dilution buffer.After reconstitution, store at -20°C to -70°C in a manual defrost freezer. A six-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 10 ng/mL is recommended.

Storage

Detection Antibody protected from prolonged exposure to light. Aliquot all other reagents and store at -20°C to -70°C in a manual defrost freezer.

Sensitivity

The minimum detectable dose of human PECAM1 was determined to be approximately 156.25 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.