Human Platelet/Endothelial Cell Adhesion Molecule, PECAM1 ELISA Kit (DEIA235)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
5 plates
Species Reactivity
Human
Intended Use
The human PECAM1 ELISA kit is for the quantitative determination of human PECAM1.
Contents of Kit
1. Capture Antibody: 0.5 mg/mL of mouseanti-PECAM1 monoclonal antibody. Dilute to a working concentration of 2.0μg/mL in CBS before coating.
2. Detection Antibody: 0.5 mg/mL rabbit anti-PECAM1 polyclonal antibody conjugated to horseradish-peroxidase (HRP).Dilute to a working concentration of 2.0μg/mL in Detection antibody dilution buffer before use.
3. Standard: Each vial contains 600 ng of recombinant PECAM1. Reconstitute with 1 mL Detection antibody Dilution buffer.
Storage
Detection Antibody should be protected from prolonged exposure to light. Aliquot all other reagentsand store at -20 to -70°C in a manual defrostfreezer. For more detailed information, please download the following document on our website.
Sensitivity
156.25pg/mL

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References


Tai, LK; Zheng, QL; et al. Flow activates ERK1/2 and endothelial nitric oxide synthase via a pathway involving PECAM1, SHP2, and Tie2. JOURNAL OF BIOLOGICAL CHEMISTRY 280:29620-29624(2005).
Dungwa, J; Ramani, P; et al. ANGIOGENIN EXPRESSION IN WILMS TUMORS CORRELATES WITH PECAM1 EXPRESSION AND CHEMOTHERAPY STATUS. PEDIATRIC BLOOD & CANCER 55:882-883(2010).

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