Animals were immunized with phytohemagglutinin-stimulated sheep T cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for sheep CD2 and precipitates a 50000 to 55000 dalton molecule from lysates of sheep lymphocytes. It does not inhibit cytotoxic T lymphocyte mediated killing or mixed lymphocyte reactivity.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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