Human T lymphocytes that had been activated with phorbol myristic acid (PMA)
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
NSI/1
Clone
16834
Cell Line Description
Animals were immunized with human T lymphocytes that had been activated with phorbol myristic acid (PMA). Spleen cells were fused with NSI/1 myeloma cells. The antibody reacts with the human T cell surface protein Tp50 (CD2). The antibody inhibits T cell proliferative responses and cytotoxicity, but does not inhibit antibody mediated cellular cytotoxicity.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 1x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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