GeneralInformation
Immunogen
Fresh peripheral blood lymphocytes from patients with expansions of gamma-delta t cells
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Described as secreting a mouse monoclonal antibody (igg2a) CD2; the cell line was established in 1987 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with fresh peripheral blood lymphocytes from patients with expansions of gamma-delta Tcells . .
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV
Morphology
single round cells growing in suspension
Growth Properties
suspension
CultureMethod
Subculturing
Split ratio: 1: 2 to 1: 3, every 3 days; seed out at 0.8 x 10^6 cells/ml; Maintain at 0.3-0.5 x 10^6 cells/ml; Doubling time: 40-50 hours; Harvest: maximal density of about 1 x 10^6 cells/ml.
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium(20%FBS), add 10%(v/v) DMSO
Target
Synonyms
CD2; CD2 molecule; CD2 antigen (p50), sheep red blood cell receptor, SRBC; T-cell surface antigen CD2; LFA-3 receptor; rosette receptor; erythrocyte receptor; lymphocyte-function antigen-2; T-cell surface antigen T11/Leu-5; CD2 antigen (p50), sheep red blood cell receptor; T11; SRBC; LFA-2; FLJ46032;
Citations
Publication ()
Have you cited CSC-H1452 in a publication?
Let us know and earn a reward for your research.
COA
Loading ......
