Human T cell leukemia cells (acute lymphoblastic leukemia)
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
P3X63Ag8.U1
Clone
PLU 22
Cell Line Description
Mice were immunized with human T cell leukemia cells (acute lymphoblastic leukemia). Spleen cells were fused with P3X63Ag8.U1 myeloma cells.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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