Sample
Serum, plasma, cell culture supernatant and other biological samples
Intended Use
In vitro quantitative determination of anti-SARS-CoV2 IgA concentrations in serum, plasma, cell culture supernatant and other biological samples.
Contents of Kit
The sealed kit can be stored at 2-8 °C. The storage condition for opened kit is specified in the table below:
1. ELISA Microplate(Dismountable), 8×12. Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
2. Lyophilized Standard, 2vial. Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 12 month at -20°C
3. HRP-labeled Antibody (Concentrated, 100×), 60μl, 2-8°C (Avoid Direct Light)
4. TMB Substrate, 5ml, 2-8°C (Avoid Direct Light)
5. Sample Dilution Buffer, 20ml, 2-8°C
6. Antibody Dilution Buffer, 5ml, 2-8°C
7. Stop Solution, 5ml, 2-8°C
8. Wash Buffer(25×), 30ml, 2-8°C
9. Plate Sealer, 5 pieces
10. Product Description, 1 copy
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.
Storage
2-8°C (for sealed box), please do not freeze! See kit label for expiry date
Performance Characteristics
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Detection Range
0.156-10ng/ml
General Description
Since commencement of COVID-19 pandemic, several SARS-CoV-2 variants have emerged . The Delta variant (B.1.617.2), discovered in October 2020. The enhanced transmissibility of Delta variant has been associated with critical mutations such as D614G, L452R, P681R, and T478K in the S-protein. As per evidence, the Delta variant possesses increased transmissibility and decreased vaccine efficacy compared to other VOCs like Alpha and Beta.
Standard Curve
This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C -25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.
Citations
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