Monkey TSH (Thyroid Stimulating Hormone)ELISA Kit

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Monkey

Detection Method

sELISA

Intended Use

In vitro quantitative determination of TSH concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

1. ELISA Microplate(Dismountable), 8×12, Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C.
2. Lyophilized Standard, 2vial, Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
3. Biotin-labeled Antibody(Concentrated, 100×), 120μl, 2-8°C (Avoid Direct Light)
4. HRP-Streptavidin Conjugate(SABC, 100×), 120μl, 2-8°C (Avoid Direct Light)
5. TMB Substrate, 10ml, 2-8°C (Avoid Direct Light)
6. Sample Dilution Buffer, 20ml, 2-8°C
7. Antibody Dilution Buffer, 10ml, 2-8°C
8. SABC Dilution Buffer, 10ml, 2-8°C
9. Stop Solution, 10ml, 2-8°C
10. Wash Buffer(25×), 30ml, 2-8°C
11. Plate Sealer, 5 pieces
12. Product Description, 1 copy
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Performance Characteristics

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

0.0156-1uIU/ml

Sensitivity

0.0094ulU/ml

General Description

Thyrotropin (TSH) is a hormone produced by thyrotropic cells in the anterior pituitary gland. TSH regulates the endocrine function of the thyroid gland. When hyperthyroidism occurs in the human body, the increase of thyroxine (T3, T4) in the body can inhibit the production and release of thyroid stimulating hormone (TSH) in the pituitary gland, so the content of thyroid stimulating hormone in the serum will be reduced at this time. Conversely, when the thyroid stimulating hormone in the human body is reduced, it indicates hyperthyroidism, and there are generally symptoms such as fear of heat and excessive sweating. Even TSH is more sensitive to the diagnosis of hyperthyroidism than T3 and T4.

Standard Curve

This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C-25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.