GeneralInformation
Immunogen
Recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the N-terminal extracellular domain (amino acids domain 19-246), alpha subunit of the human thyroid stimulating hormone (TSH) receptor (hormone binding unit). They recognize different epitopes.It cross reacts with the rodent TSHR. The antibodies do not interfere with hormone binding and have no effect on the receptor mediated adenylate cyclase activity.
Application Notes
WB, ELISA, ICC, and IHC of the human TSH receptor.
Growth Properties
Suspension, with some loosely Adherent cells.
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
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COA
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