Recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0
Clone
301-5F21
Cell Line Description
Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the N-terminal extracellular domain (domain amino acids 19-246), alpha subunit of the human thyroid stimulating hormone (TSH) receptor (hormone binding unit).
Application Notes
WB, ELISA, ICC, and IHC of the human TSH receptor.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 3-5 X 10^4 cells/mL and maintain cultures between 3x10^4 - 4x10^5 cells/ml
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