Recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0
Clone
UTIS-U4-476
Cell Line Description
Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the C-terminal intracellular domain (amino acids domain 604-764), beta subunit of the human thyroid stimulating hormone (TSH) receptor. It recognizes an intracellular epitope of the beta subunit anchored into the cellular membrane.
Application Notes
WB, ELISA, ICC, and IHC of the human TSH receptor.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 2x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
Publication ()
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