GeneralInformation
Immunogen
Recombinant human thyroid stimulating hormone (TSH) receptor
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the N-terminal extracellular domain (amino acids domain 19-246), alpha subunit of the human thyroid stimulating hormone (TSH) receptor (hormone binding unit). The antibody do not interfere with hormone binding and have no effect on the receptor mediated adenylate cyclase activity. The antibodycan be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor.
Application Notes
WB, ELISA, ICC, and IHC of the human TSH receptor.
Growth Properties
Suspension (some Adherent cells)
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
Citations
Publication ()
Have you cited CSC-H0530 in a publication?
Let us know and earn a reward for your research.
COA
Loading ......
