RHA™ anti-Leucomalachite green monoclonal antibody, clone LMG

The nitrogen-fixing bacterial strain UFLA 01-1174(T)was isolated from nodules ofCampsiandra laurilifoliaBenth. originating from the Amazon region, Brazil. Its taxonomic position was defined using a polyphasic approach. Analysis of the 16S rRNA gene placed the strain in theBradyrhizobiumgenus, the closest species beingB. guangdongenseCCBAU 51649(T)andB. guangzhouenseCCBAU 51670(T), both with 99.8% similarity. Multilocus sequence analysis (MLSA) ofrecA,gyrB,glnII,rpoB,atpD, anddnaKindicated that UFLA 01-1174(T )is a new species, most closely related toB. stylosanthisBR 446(T)(94.4%) andB. manausenseBR 3351(T)(93.7%). Average nucleotide identity (ANI) differentiated UFLA 01-1174(T)from the closest species with values lower than 90%. The G + C content in the DNA of UFLA 01-1174(T )is 63.6 mol%. Based on this data, we conclude that the strain represents a new species. The name proposed isBradyrhizobium campsiandrae, with UFLA 01-1174(T)(= INPA 394B(T) = LMG 10099(T)) as type strain.

The low-molecular-weight gelator (LMG) 12-hydroxyoctadecanoic acid (12-HOA) is insoluble in water, but can be solubilized in surfactant micelles. We therefore solubilized 12-HOA at 80 degrees C in an aqueous solution of cetyltrimethylammonium bromide (CTAB) containing spherical micelles. On cooling this system down to room temperature, a hydrogel is obtained. We will refer to this process as "surfactant-mediated gelation" (SMG). The hydrogels were formed at a lower 12-HOA concentration when sodium salicylate (NaSal) was added to the CTAB system, which induced the formation of wormlike micelles. Hydrogels obtained by SMG from spherical and wormlike micelles are referred to as gelled micellar phases (GMs) and gelled wormlike micellar phases (GWLMs), respectively. Optical microscopy and transmission electron microscopy (TEM) showed that 12-HOA forms self-assembled fibrillar networks (SAFiNs) in both GMs and GWLMs. The sol-gel transition temperature, Tsol-gel, of the GWLM samples was higher than that of the GM samples. Dynamic rheological measurements revealed gel properties (G ' > G '' at all angular frequencies) for both gels; however, a higher viscoelasticity was observed for the GWLM samples, which in turn, was reflected in the higher Tsol-gel. Small- and wide-angle X-ray scattering (SWAXS) showed that micelles and gel fibers coexist in the GM and GWLM samples. Our study demonstrates the gelation of aqueous micellar solutions with water-insoluble LMGs.