RHA™ anti-Malachite green monoclonal antibody, clone MG

A Gram-stain-negative, facultatively anaerobic, motile, light-yellow, and rod-shaped bacterium, designated as NY-02(T), was isolated from the sludge of a wastewater treatment plant in Hanam City, Republic of Korea. The comparison of 16S rRNA gene sequences revealed that the strain formed a distinct lineage within the genus Simplicispira and was most closely related to S. suum SC1-8(T) (99.0%), S. limi EMB325(T) (98.3%), S. psychrophila LMG 5408(T) (98.2%), and S. piscis RSG39(T) (97.4%). Both average nucleotide identity (ANI) and DNA-DNA hybridization (DDH) values between strain NY-02(T), and its closes type strains [S. suum SC1-8(T), S. limi EMB325(T), S. psychrophila LMG 5408(T), and S. piscis RSG39(T)] were lower than the cut-off (>= 95-96% for ANI and >= 70% for DDH) to define a bacterial species. The genome comprises of 3,709,074 bp with a G + C content of 64.2 mol%. Ubiquinone 8 (Q-8) was the predominant quinone. The major fatty acids were C-16:0 and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), and the major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylethanolamine. The results of the physiological, biochemical, and taxonomic analyses in addition to low ANI and DNA-DNA relatedness values (82.2% and < 34.0%, respectively) indicate that the strain NY-02(T) represents a novel species of the genus Simplicispira. The name proposed for strain NY-02(T) (= KACC 19731(T) = LMG 31165(T)) is Simplicispira hankyongi sp. nov.

In the present study, ZnFe2O4@methyl cellulose (MC) nano-biomagnetic photocatalyst was rapidly prepared based on a microwave-assisted method. FTIR, FESEM, EDS, UV-DRS, XRD, and VSM were performed to characterize the structure of as-prepared ZnFe2O4@MC. The removal efficiency of Metronidazole (MNZ) degradation was 92.65% and 71.12% in synthetic and real samples under optimal conditions, respectively. The removal efficiency of TOC was also reported to be 77.87% under optimal conditions. The kinetic linear models showed that the photocatalytic degradation of MNZ follows either a pseudo-first-order kinetic or the Langmuir-Hinshelwood model. The correlation coefficients (R-2) were 0.92, 0.97, 0.99, and 0.94, respectively at 5, 10, 20, and 30 mg/L. The equilibrium adsorption coefficient (KL-H) of the Langmuir-Hinshelwood model and the superficial reaction rate constant (K-c) were 0.633 Lmg(-1) and 0.203 mg/L min(-1), respectively. The participation of active species such as holes and hydroxyl and superoxide radicals was studied during MNZ photodegradation with organic and inorganic radical scavengers. Finally, the nano-biomagnetic catalyst could be reused for six further runs without remarkable changes in catalytic efficiencies. In this study, we present a new magnetic nanocomposite and a novel strategy for antibiotic removal from aqueous media. (C) 2020 Elsevier B.V. All rights reserved.