Malachite Green ELISA Kit (DEIA057)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
animal tissue, water
Species Reactivity
N/A
Intended Use
This kit can be used in quantitative and qualitative analysis of malachite green residue in animal tissue (fish and shrimp), water etc.
Storage
Storage condition: 2-8°C.
Storage period: 12 months.
Precision
Variation coefficient of the ELISA kit is less than 10%.
Detection Limit
Tissue ( fish, shrimp): 0.12ppb
Water: 0.2ppb
Sensitivity
0.02ppb
General Description
Malachite green is an organic compound that is used as a dyestuff and has emerged as a controversial agent in aquaculture. Fishes and shrimp contaminated by malachite green may cause health problems in human after being consumed. Strict MRLs have been established against malachite green in EU, US and Japan, etc.
This kit is a new product based on ELISA technology, which is fast (only 45min in one operation), easy, accurate and sensitive compared with common instrumental analysis, and so it can considerably minimize operation error and work intensity.

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References


Direct immobilization of triphenylphosphine palladium complexes on the external surface of zeolite beta

MICROPOROUS AND MESOPOROUS MATERIALS

Authors: Okumura, Kazu; Sato, Kakeru; Kamioka, Keisuke; Koga, Yuito

The immobilization of a Pd complex on several supports was attempted using a simple method. For this purpose, bis(triphenylphosphine)palladium(II) dichloride(Pd(PPh3)(2)Cl-2) was attached to several oxides including MgO, gamma-Al2O3, TiO2, and zeolites. Pd(PPh3)(2)Cl-2 supported on zeolite beta remained mostly intact, whereas the complex completely decomposed to provide metal Pd or PdO on MgO and gamma-Al2O3, as analyzed by Pd K-edge X-ray absorption fine structure spectroscopy (XAFS). The specific surface area was slightly reduced after Pd(PPh3)(2)Cl-2 loading on zeolite beta, probably due to the deposition of the complex on the external surface. From a comparison experiment using the adsorption of triphenylphosphine (PPh3), it was supposed that the PPh3 ligand of Pd(PPh3)(2)Cl-2 was inserted into the pores of zeolite beta located close to the external surface. The Pd complexes immobilized on zeolite beta exhibited much higher activity for allylic alkylation compared with that of pristine Pd(PPh3)(2)Cl-2. Recycling of the zeolite beta loaded Pd complex was possible after the used catalyst was rinsed with toluene. The activity of the Pd complex loaded on zeolite beta was enhanced by the addition of a small amount of leucomalachite green (LMG) or leucocrystal violet (LCV).

Enterococcus pingfangensis sp. nov., Enterococcus dongliensis sp. nov., Enterococcus hulanensis sp. nov., Enterococcus nangangensis sp. nov. and Enterococcus songbeiensis sp. nov., isolated from Chinese traditional pickle juice

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

Authors: Li, Yu Qin; Gu, Chun Tao

Five novel lactic acid bacterial strains, isolated from Chinese traditional pickle juice, were characterised by a polyphasic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, determination of DNA G+C content, average nucleotide identity (ANI) analysis, in silico DNA-DNA hybridisation (isDDH), fatty acid methyl ester (FAME) analysis and an analysis of phenotypic features. Strains 241-2-2(T), 63-4(T) and 190-7(T) were closely related to the type strains of Enterococcus devriesei, Enterococcus viikkiensis, Enterococcus pseudoavium, Enterococcus xiangfangensis, Enterococcus avium, Enterococcus malodoratus, Enterococcus raffinosus and Enterococcus gilvus, having 99.1-99.9% 16S rRNA gene sequence similarities. Strain 94-2(T) was distantly related to the type strains of Enterococcus phoeniculicola, Enterococcus rivorum and Enterococcus faecalis, having 95.2-96.1% 16S rRNA gene sequence similarities. Strain 85-4(T) was distantly related to the type strains of Enterococcus casseliflavus, Enterococcus gallinarum, Enterococcus dispar, Enterococcus canintestini, Enterococcus saigonensis, Enterococcus diestrammenae, Enterococcus asini, Enterococcus cecorum and Enterococcus columbae, having 95.7-97.8% 16S rRNA gene sequence similarities. Less than 91.5% ANI and 45.3% isDDH values between strains 241-2-2(T), 63-4(T), 190-7(T), 94-2(T), 85-4(T) and type strains of phylogenetically related species showed that they represent five new species within the genus Enterococcus. Based upon the data of polyphasic characterisation obtained in the present study, five novel species, Enterococcus pingfangensis sp. nov., Enterococcus dongliensis sp. nov., Enterococcus hulanensis sp. nov., Enterococcus nangangensis sp. nov. and Enterococcus songbeiensis sp. nov., are proposed and the type strains are 241-2-2(T) (=NCIMB 15185(T) = CCM 8921(T) = LMG 31181(T)), 63-4(T) (=NCIMB 15178(T) = CCM 8922(T)), 190-7(T) (=NCIMB 15200(T) = CCM 8949(T)), 94-2(T) (=NCIMB 15180(T) = CCM 8920(T) = LMG 31180(T)) and 85-4(T) (=NCIMB 15179(T) = CCM 8923(T) = LMG 31183(T)), respectively.

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