Leucomalachite Green [HRP]

Root-nodule bacteria were isolated from wild legumes growing in the Kavango region, Namibia. Using a polyphasic approach, four strains belonging to the genus Bradyrhizobium (WR4(T), WR87, T10 and T12) were further characterized to clarify the taxonomic status of this group. On the basis of 16S rRNA gene sequences, the four strains showed highest similarity to Bradyrhizobium elkanii USDA 76(T) (99.9 %), Bradyrhizobium pachyrhizi PAC48(T) (identical) and to Bradyrhizobium brasilense UFLA03-321(T) (identical). Multilocus sequence analysis of concatenated glnll-recA-gyrB-dnaK-rpoB sequences and comparison of the intergenic transcribed spacer (ITS) sequences confirmed that the novel group belongs to a distinct lineage of the genus Bradyrhizobium, with <96.7 % (MLSA) and 97.25 % (ITS) nucleotide identity with B. elkanii USDA 76(T). Results from the sequence-based analysis were validated by DNA-DNA hybridization experiments and suggested a novel species. Several phenotypic features including carbon compound utilization and growth characteristics supported the phylogenetic data, thus it is concluded that the strains represent a novel species, for which the name Bradyrhizobium ripae sp. nov. is proposed, with type strain WR4(T) [LMG 30283, DSM 105795, NTCCM 0019 (Windhoek)].

The success of construction projects is largely influenced by the significant costs incurred by workplace deviant behavior (WDB). It is useful to better understand how WDB occurs among workers for its preventive management. By invoking social exchange theory, this paper clarifies how WDB among construction workers can be predicted by leader-member exchange (LMX) and leader-member guanxi (LMG). Data were gathered from 153 workers and 17 supervisors in 17 construction projects. The results show that LMX and LMG are negatively related to WDB. LMG moderates the relationship between LMX and WDB: The negative relationship between LMX and WDB is stronger when LMG has higher quality. The findings contribute to research on WDB, especially from the aspect of leader-member relationship in the construction industry, and shed light on the theory of social exchange by capturing the role of its nonwork part, LMG, in predicting WDB. Managers of construction projects can benefit from the novel explanation offered here of the occurrence of WDB among workers and suggestions on controlling it in advance.