Intended Use
Allergen specific IgE ELISA Kit is for in vitro qualititative measurement of IgE in human serum.
The inhalant allergen includes Dermatophagoides pteronyssinus, house dust, cockroach, cat epithelium, dog epithelium, moulds, tree pollens, white mulberry and short ragweed.
Contents of Kit
1. ELISA microplate 1pc
2. Anti-Human IgE Antibody-Biotin 2 x 5mL
3. Streptavidin-HRP 2 x 5mL
4. Concentrated Wash Buffer 2 x 25mL
5. Stop Solution 1 x 6mL
6. Substrate A 1 x 6mL
7. Substrate B 1 x 6mL
8. Sample Buffer 1 x 6mL
9. IgE Positive Control 1 x 1.3mL
10. IgE Negative Control 1 x 1.3mL
Precision
Intra-assay CV: ≤15%
Inter-assay CV: ≤15%
Detection Limit
Allergen: 0.35IU/mL
Total IgE: 3 IU/Ml
General Description
The existence of IgE in man as a unique class of immunoglobulins which are important in the mediation of the allergic response has been known for over twenty years. The mechanism of action involves an initial antigenic stimulation of immunocompetent B lymphocytes by a specific antigen, a process which induces the lymphocyte to respond by producing specific antibody of several classes.
Citations
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