Food Allergies IgG - Allerquant 90G ELISA Kit (DEIA2264)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Species Reactivity
Intended Use
The 90 Food IgG Elisa Test is for measuring the relative amount of food-specific IgG antibody in human serum.
Contents of Kit
1. Food Extract Coated Microwell Plates: 3 plates
2. Sample Diluent (Green): 1 x 56 mL
3. Wash Buffer (concentrate): 1 x 30 mL
4. Food IgG Calibrator: 1.0 mL
5. Food IgG Positive Control: 1.0 mL
6. Food IgG-HRP Conjugate: 1 x 40 mL
7. Substrate Solution A (TMB): 2 x 12 mL
8. Substrate Solution B (hydrogen peroxide): 2 x 12 mL
9. Stopping Solution (1N H2SO4): 1 x 20 mL
For more detailed information, please download the following document on our website.
Detection Range
50-400 U/mL


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Immunoglobulins G modulate endothelial function and affect insulin sensitivity in humans


Authors: Napoli, Raffaele; Ruvolo, Antonio; Triggianese, Paola; Prevete, Nella; Schiattarella, Gabriele G.; Nigro, Cecilia; Miele, Claudia; Magliulo, Fabio; Grassi, Simona; Pecoraro, Antonio; Cittadini, Antonio; Esposito, Giovanni; de Paulis, Amato; Spadaro, Giuseppe

Background and aims: Data from animals suggest that immunoglobulins G (IgG) play a mechanistic role in atherosclerosis and diabetes through endothelial dysfunction and insulin resistance. Patients with common variable immunodeficiency (CVID), who have low circulating levels of IgG and are treated with intravenous polyclonal IgG (IVIgG), may provide an ideal model to clarify whether circulating IgG modulate endothelial function and affect insulin sensitivity in humans. Methods and results: We studied 24 patients with CVID and 17 matched healthy controls (HC). Endothelial function was evaluated as flow mediated dilation (FMD) of the brachial artery at baseline and 1, 7, 14, and 21 days after IVIgG infusion in the CVID patients. We measured also plasma glucose, insulin, and calculated the HOMA-IR index. We also investigated the role of human IgG on the production of Nitric Oxide (NO) in vitro in Human Coronary Artery Endothelial Cells (HCAEC). Compared to HC, FMD of CVID patients was significantly impaired at baseline (9.4 +/- 0.9 and 7.6 +/- 0.6% respectively, p < 0.05) but rose above normal levels 1 and 7 days after IVIgG infusion to return at baseline at 14 and 21 days. Serum insulin concentration and HOMA-IR index dropped by 50% in CVID patients after IVIgG (p < 0.002 vs. baseline). In vitro IgG stimulated NO production in HCAEC. Conclusions: Reduced IgG levels are associated with endothelial dysfunction and IVIgG stimulates endothelial function directly while improving insulin sensitivity. The current findings may suggest an anti-atherogenic role of human IgG. (C) 2020 The Italian Diabetes Society, the Italian Society for the Study of Atherosclerosis, the Italian Society of Human Nutrition and the Department of Clinical Medicine and Surgery, Federico II University. Published by Elsevier B.V. All rights reserved.

Characterization of adult patients with IgG subclass deficiency and subnormal IgG2


Authors: Barton, James C.; Barton, Jackson C.; Bertoli, Luigi F.; Acton, Ronald T.

Background Adults with IgG subclass deficiency (IgGSD) with subnormal IgG2 are inadequately characterized. Methods We retrospectively analyzed observations in unrelated adults with IgGSD evaluated in a single hematology clinic (1991-2019) and selected those with subnormal serum IgG2 (<117 mg/dL (<1.2 g/L)) without corticosteroid therapy to describe: age; prevalence of women; upper/lower respiratory infection; autoimmune condition(s); atopy; other allergy; frequent or severe respiratory tract infection in first-degree relatives; IgG, IgG subclasses, IgA, and IgM; blood lymphocyte subpopulations; human leukocyte antigen (HLA)-A and -B types and haplotypes; and 23-valent pneumococcal polysaccharide vaccination (PPSV23) responses. We determined the prevalence of subnormal IgG2 among unrelated adults with IgGSD without corticosteroid therapy and compared general characteristics of those with and without subnormal IgG2. Results There were 18 patients (94.4% women) with subnormal IgG2. Mean age was 52 +/- 11 y. Upper/lower respiratory infection occurred in 94.4%/74.8%, respectively. Autoimmune condition(s), atopy, other allergy, and frequent or severe respiratory infection in first-degree relatives occurred in 44.4%, 44.4%, 61.1%, and 22.2%, respectively. Median IgG2 was 105 mg/dL (83, 116). Subnormal IgG, IgG1, IgG3, IgG4, IgA, and IgM was observed in 66.7%, 50.0%, 100.0%, 5.6%, 33.3%, and 0%, respectively. Lymphocyte subpopulations were normal in most patients. HLA frequencies were similar in patients and controls. Three of 4 patients had no protectiveS.pneumoniaeserotype-specific IgG levels before or after PPSV23. These 18 patients represent 7.6% of 236 adults with IgGSD. Prevalence of subnormal IgG, subnormal IgG3, and subnormal IgA was significantly greater in 18 adults with subnormal IgG2 than 218 adults without subnormal IgG2. Prevalence of subnormal IgM was significantly lower in patients with subnormal IgG2. Conclusions Characteristics of adults with IgGSD with subnormal IgG2 include female predominance, other immunologic abnormalities, subnormal IgG3 and/or IgG1, lack of HLA-A and -B association, and suboptimal PPSV23 response.

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