IgG Screen Nutritional 88 ELISA Kit

Name: IgG Screen Nutritional 88 ELISA Kit
SKU: DEIA302
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum and plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The IgG Screen Nutritional 88 ELISA test kit has been designed for the detection and the quantitative determination of specific food antigen-related IgG antibodies in serum and plasma. Further applications in other body fluids are possible and can be requested from the Technical Service. This assay is intended for research use only.

Contents of Kit

The IgG Screen Nutritional 88 ELISA kit contains sufficient reagents for the testing of one sample (88 determinations) and controls as well as generation of a calibration curve. The strips and solutions have to be stored at 2-8°C. The expiry date is mentioned on the labels.
Microtiter Strips: 1 microtiter plate coated with 88 different nutritional antigens (see distribution scheme) and reference antigen (1^st strip, color coded violet). Ready-to-use.
Standards: 6×0.5 mL, human plasma diluted with PBS/BSA, with 0.35, 0.7, 3.5, 17.5, 50 and 100 U/mL of IgG antibodies to egg white. Addition of 0.05% sodium azide. Ready-to-use.
Low Positive Control: 1×0.5 mL, human plasma including low concentrations of IgG antibodies. Addition of 0.05% sodium azide.
High Positive Control: 1×0.5 mL, human plasma including high concentrations of IgG antibodies. Addition of 0.05% sodium azide.
Sample Diluent: 40 mL, Tris/BSA buffer. Addition of 0.05% sodium azide. Ready-to-use.
Conjugate: 15 mL, mouse-a-human-IgG-AP, in proteinacious buffer solution. Ready-to-use.
Substrate: 15 mL, Paranitrophenylphosphate (PNPP), Ready-to-use.
Stop Solution: 15 mL, 1 M sodium hydroxide. Ready-to-use.
Washing Buffer: 60 mL, PBS + Tween 20, 10× concentrate. Final concentration: dilute 1+9 with distilled water. If during the cold storage crystals precipitate, the concentrate should be warmed up at 37°C for 15 minutes.
Plastic Foils: 2 pieces to cover the microtiter strips during the incubation.
Instruction Booklet
Distribution Scheme

Storage

Store kit at 2-8°C. It is stable up to the expiry date stated on the label of the box. Do not use kit beyond its expiry date.

Performance Characteristics

General Description

Incompatibility reactions against food may cause various symptoms in the human organism and this disturbance is manifested in the immune system by the formation of specific IgE as well as IgG and/or IgG4 antibodies. Statistics show that 60% of the population suffer from intolerances against at least one foodstuff, which may cause clinical symptoms or enhance them. Hints may be various and reach from skin irritations over digestive disorders up to migraine. With the diagnostic findings of unspecific discomfort, allergies or intolerances against food should be clarified. The theoretical basis for the determination of specific IgG or IgG4 for the diagnosis of food intolerances depends on the observation that some subclasses of IgG (mainly IgG4) are connected to the in vitro degranulation of basophilic cells and mastocytes and the activation of the complement cascade. It was also observed, that high concentrations of circulating IgG were measured in atopic persons. Already early surveys showed that in persons with inflammatory reactions against food IgG but not IgE was detected. Significantly enhanced IgG and IgG4 titers were also found in patients with food intolerances. Skin tests are relatively poorly correlated to food allergies and are only significant in the presence of IgE related reactions. As additional diagnostic tools provocation and elimination diets are applied. These methods depend strongly on the motivation and compliance of the patient. Due to these constraints nowadays serological determinations of antibodies against various food panels are applied increasingly. The two reactions related with the immune system differ insofar as the IgE associated food allergy occurs within the next hour following the food intake, while IgG/IgG4 intolerances show a delayed reaction of 24 to 120 hours and persistent symptoms may arise.

Citations

Have you cited DEIA302 in a publication? Let us know and earn a reward for your research.

Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

A compromised specific humoral immune response against the SARS-CoV-2 receptor-binding domain is related to viral persistence and periodic shedding in the gastrointestinal tract

CELLULAR & MOLECULAR IMMUNOLOGY

Authors: Hu, Fengyu; Chen, Fengjuan; Ou, Zhihua; Fan, Qinghong; Tan, Xinghua; Wang, Yaping; Pan, Yuejun; Ke, Bixia; Li, Linghua; Guan, Yujuan; Mo, Xiaoneng; Wang, Jian; Wang, Jinlin; Luo, Chun; Wen, Xueliang; Li, Min; Ren, Peidi; Ke, Changwen; Li, Junhua; Lei, Chunliang; Tang, Xiaoping; Li, Feng

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been redetected after discharge in some coronavirus disease 2019 (COVID-19) patients. The reason for the recurrent positivity of the test and the potential public health concern due to this occurrence are still unknown. Here, we analyzed the viral data and clinical manifestations of 289 domestic Chinese COVID-19 patients and found that 21 individuals (7.3%) were readmitted for hospitalization after detection of SARS-CoV-2 after discharge. First, we experimentally confirmed that the virus was involved in the initial infection and was not a secondary infection. In positive retests, the virus was usually found in anal samples (15 of 21, 71.4%). Through analysis of the intracellular viral subgenomic messenger RNA (sgmRNA), we verified that positive retest patients had active viral replication in their gastrointestinal tracts (3 of 16 patients, 18.7%) but not in their respiratory tracts. Then, we found that viral persistence was not associated with high viral titers, delayed viral clearance, old age, or more severe clinical symptoms during the first hospitalization. In contrast, viral rebound was associated with significantly lower levels of and slower generation of viral receptor-binding domain (RBD)-specific IgA and IgG antibodies. Our study demonstrated that the positive retest patients failed to create a robust protective humoral immune response, which might result in SARS-CoV-2 persistence in the gastrointestinal tract and possibly in active viral shedding. Further exploration of the mechanism underlying the rebound in SARS-CoV-2 in this population will be crucial for preventing virus spread and developing effective vaccines.

IgG Immune Complexes Break Immune Tolerance of Human Microglia

JOURNAL OF IMMUNOLOGY

Authors: van der Poel, Marlijn; Hoepel, Willianne; Hamann, Jorg; Huitinga, Inge; den Dunnen, Jeroen

Abstract

Microglia are phagocytic cells involved in homeostasis of the brain and are key players in the pathogenesis of multiple sclerosis (MS). A hallmark of MS diagnosis is the presence of IgG Abs, which appear as oligoclonal bands in the cerebrospinal fluid. In this study, we demonstrate that myelin obtained post mortem from 8 out of 11 MS brain donors is bound by IgG Abs. Importantly, we show that IgG immune complexes strongly potentiate activation of primary human microglia by breaking their tolerance for microbial stimuli, such as LPS and Poly I:C, resulting in increased production of key proinflammatory cytokines, such as TNF and IL-1 beta. We identified Fc gamma RI and Fc gamma RIIa as the two main responsible IgG receptors for the breaking of immune tolerance of microglia. Combined, these data indicate that IgG immune complexes potentiate inflammation by human microglia, which may play an important role in MS-associated inflammation and the formation of demyelinating lesions.