IgG Screen Nutritional ELISA Kit

Name: IgG Screen Nutritional ELISA Kit
SKU: DEIA301
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum and plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The IgG Screen Nutritional 24 ELISA test kit has been designed for the detection and the quantitative determination of specific food antigen-related IgG antibodies in serum and plasma. Further applications in other body fluids are possible and can be requested from the Technical Service. This assay is intended for research use only.

Contents of Kit

The IgG Screen Nutritional 24 ELISA kit contains one microtiter plate and sufficient reagents for the testing of 3 samples (24 determinations per sample) as well as the generation of 3 standard curves and the testing of controls. The strips and solutions have to be stored at 2-8°C. The expiry date is mentioned on the labels.
Microtiter Strips: For 3 samples with 4 color coded strips with 8 wells each. There are 4 different strips (coded with violet, green, yellow and red color) required per screen (sample). 8× reference antigen is bound on the violet strip. 24 (3×8) food antigens are bound on the green, yellow and red strips. See attached distribution scheme. Ready to use.
Standards: 6×0.5 mL, human plasma diluted with PBS/BSA, with 0.35, 0.7, 3.5, 17.5, 50 and 100 U/mL of IgG antibodies to egg white. Addition of 0.05% sodium azide. Ready to use.
Low Positive Control: 1 x 0.5 mL, human plasma including low concentrations of IgG antibodies. Addition of 0.05% sodium azide.
High Positive Control: 1 x 0.5 mL, human plasma including high concentrations of IgG antibodies. Addition of 0.05% sodium azide.
Sample Diluent: 40 mL, Tris/BSA buffer. Addition of 0.05% sodium azide. Ready to use.
Conjugate: 15 mL, mouse-a-human-IgG-AP, in proteinacious buffer solution. Ready to use.
Substrate: 15 mL, Paranitrophenylphosphate (PNPP), Ready to use.
Stop Solution: 15 mL, 1 M sodium hydroxide. Ready to use.
Washing Buffer: 60 mL, PBS + Tween 20, 10× concentrate. Final concentration: dilute 1+9 with distilled water. If during the cold storage crystals precipitate, the concentrate should be warmed up at 37°C for 15 minutes.
Plastic Foils: 2 pieces to cover the microtiter strips during the incubation.
Instruction Booklet
Distribution Scheme

Storage

Store kit at 2-8°C. It is stable up to the expiry date stated on the label of the box. Do not use kit beyond its expiry date.

Performance Characteristics

General Description

Incompatibility reactions against food may cause various symptoms in the human organism and this disturbance is manifested in the immune system by the formation of specific IgE as well as IgG and/or IgG4 antibodies. Statistics show that 60% of the population suffer from intolerances against at least one foodstuff, which may cause clinical symptoms or enhance them. Hints may be various and reach from skin irritations over digestive disorders up to migraine. With the diagnostic findings of unspecific discomfort, allergies or intolerances against food should be clarified. The theoretical basis for the determination of specific IgG or IgG4 for the diagnosis of food intolerances depends on the observation that some subclasses of IgG (mainly IgG4) are connected to the in vitro degranulation of basophilic cells and mastocytes and the activation of the complement cascade. It was also observed, that high concentrations of circulating IgG were measured in atopic persons. Already early surveys showed that in persons with inflammatory reactions against food IgG but not IgE was detected. Significantly enhanced IgG and IgG4 titers were also found in patients with food intolerances. Skin tests are relatively poorly correlated to food allergies and are only significant in the presence of IgE related reactions. As additional diagnostic tools provocation and elimination diets are applied. These methods depend strongly on the motivation and compliance of the patient. Due to these constraints nowadays serological determinations of antibodies against various food panels are applied increasingly. The two reactions related with the immune system differ insofar as the IgE associated food allergy occurs within the next hour following the food intake, while IgG/IgG4 intolerances show a delayed reaction of 24 to 120 hours and persistent symptoms may arise.

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Long-term effects of intensive B cell depletion therapy in severe cases of IgG4-related disease with renal involvement

IMMUNOLOGIC RESEARCH

Authors: Quattrocchio, Giacomo; Barreca, Antonella; Demarchi, Andrea; Fenoglio, Roberta; Ferro, Michela; Del Vecchio, Giulio; Massara, Carlo; Rollino, Cristiana; Sciascia, Savino; Roccatello, Dario

Abstract

IgG4-related disease (IgG4-RD) is an immune-mediated disorder often showing elevated serum IgG4 concentrations, dense T and B lymphocyte infiltration, and IgG4-positive plasma cells and storiform fibrosis. We prospectively evaluated for 4 years 5 patients with histologically proven IgG4-RD of whom 3 had tubulointerstitial nephritis (TIN) and 2 had retroperitoneal fibrosis (RPF). They received an intensive B depletion therapy with rituximab. The estimated glomerular filtration rate of TIN patients after 1 year increased from 9 to 24 ml/min per 1.73 m2. IgG/IgG4 dropped from 3236/665 to 706/51 mg/dl, C3/C4 went up from 49/6 to 99/27 mg/dl, and the IgG4-RD responder index fell from 10 to 1. CD20(+) B cells decreased from 8.7 to 0.5%. A striking drop in interstitial plasma cell infiltrate as well as normalization of IgG4/IgG-positive plasma cells was observed at repeat biopsy. Both clinical and immunological improvement persisted over a 4-year follow-up. Treating these patients who were affected by aggressive IgG4-RD with renal involvement in an effort to induce a prolonged B cells depletion with IgG4 and cytokine production decrease resulted in a considerable rise in eGFR, with IgG4-RD RI normalization and a noteworthy improvement in clinical and histological features. Furthermore, the TIN subgroup was shown not to need for any maintenance therapy.

Glycosylation Alterations in Multiple Sclerosis Show Increased Proinflammatory Potential

BIOMEDICINES

Authors: Cvetko, Ana; Kifer, Domagoj; Gornik, Olga; Klaric, Lucija; Visser, Elizabeth; Lauc, Gordan; Wilson, James F.; Stambuk, Tamara

Abstract

Multiple sclerosis (MS) is an inflammatory autoimmune disorder affecting the central nervous system (CNS), with unresolved aetiology. Previous studies have implicated N-glycosylation, a highly regulated enzymatic attachment of complex sugars to targeted proteins, in MS pathogenesis. We investigated individual variation in N-glycosylation of the total plasma proteome and of IgG in MS. Both plasma protein and IgG N-glycans were chromatographically profiled and quantified in 83 MS cases and 88 age- and sex-matched controls. Comparing levels of glycosylation features between MS cases and controls revealed that core fucosylation (p = 6.96 x 10(-3)) and abundance of high-mannose structures (p = 1.48 x 10(-2)) were the most prominently altered IgG glycosylation traits. Significant changes in plasma protein N-glycome composition were observed for antennary fucosylated, tri- and tetrasialylated, tri- and tetragalactosylated, high-branched N-glycans (p-value range 1.66 x 10(-2)-4.28 x 10(-2)). Classification performance of N-glycans was examined by ROC curve analysis, resulting in an AUC of 0.852 for the total plasma N-glycome and 0.798 for IgG N-glycome prediction models. Our results indicate that multiple aspects of protein glycosylation are altered in MS, showing increased proinflammatory potential. N-glycan alterations showed substantial value in classification of the disease status, nonetheless, additional studies are warranted to explore their exact role in MS development and utility as biomarkers.