IgG4 Screen Nutritional 88 ELISA Kit (DEIA304)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
96T
Sample
serum, plasma
Species Reactivity
Human
Intended Use
The IgG4 Screen Nutritional 88 ELISA test kit has been designed for the detection andthe quantitative determination of specific food antigenn-related IgG4 antibodies in serum and plasma.
Contents of Kit
1. Microtiter Strips
2. Standards
3. Low positive Control
4. High positive Control
5. Sample Diluent
6. Conjugate
7. Substrate
8. Stop Solution
9. Washing Buffer
Storage
For more detailed information, please download the following document on our website.
Sensitivity
0.22 U/mL

Citations


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References


Validation and cross-reactivity pattern assessment of monoclonal antibodies used for the screening of donor-specific IgG antibody subclasses in transplant recipients

JOURNAL OF IMMUNOLOGICAL METHODS

Authors: Jucaud, Vadim; Anh Nguyen; Bach Tran; Hopfield, Judy; Tho Pham

The screening for IgG subclass donor-specific antibodies (DSAs) in allograft recipients uses IgG1-4 subclass-specific monoclonal antibodies (mAbs) that should be mono-specific. The cross-reactivity discrepancies reported for IgG subclass-specific mAbs warranted a critical cross-reactivity pattern analysis of the IgG subclass-specific mAbs most commonly used to detect DSAs. We tested the reactivity of 2 anti-IgG1-, 3 anti-IgG2-, 1 anti-IgG3-, and 2 anti-IgG4-specific PE-conjugated mAbs against microbeads coated with IgG1-4 proteins separately. Each IgG subclass protein was coated at three densities on the beads (0.5, 1, and 2 pg of protein per 10(6) beads), and the PE-conjugated mAbs were titrated from 0.04 mu g/mL to 5 mu g/mL. The IgG subclass reactivity of the sample was acquired on the Luminex multiplex platform. Among the IgG subclass-specific mAbs, only the anti-IgG3 (clone: HP6050) mAb was mono-specific. All other mAbs tested were binding to IgG subclass proteins other than their respective immunogen, thereby being cross-reactive. IgG subclass cross-reactivity patterns were dependent on the concentration of both IgG subclass-specific mAbs and IgG1-4 protein targets coated onto the beads. With the current IgG subclass mAbs available, 3 of the 15 possible combinations of IgG1-4 subclass protein could be identified. While the remaining 12 unique combinations cannot be distinguished clearly, 6 groups that corresponded to two different unique combinations of IgG1-4 subclass protein could be identified. The dilution of serum samples and IgG subclass-specific mAbs, other than the anti-IgG3 (clone: HP6050), must be further optimized before their implementation in IgG subclass DSA screening in allograft recipients.

Seroprevalence of Dengue virus among pregnant mothers and their-newborn infants in the southwest of Saudi Arabia

CLINICAL AND EXPERIMENTAL OBSTETRICS & GYNECOLOGY

Authors: Alqahtani, Ali S.

Dengue virus (DENV) is one of the most serious arthropod-borne viruses. This study was performed to investigate the prevalence of the virus in Najran, a city in the south west of Saudi Arabia. Four hundred and ten paired maternal and umbilical serum samples were obtained from mothers and their newborn infants. The samples were tested for the presence of IgG antibodies by ELISA. Anti-DENV IgG antibodies were detected in 31 maternal samples (7.6%), while all newborn samples were negative. All maternal seropositive samples were negative when tested by PCR. These results are further indication that DENV is endemic in Saudi Arabia and enhanced DENV surveillance is necessary.

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