Human anti-EPO antibody(anti-Erythropoietin antibody) ELISA Kit

Sample

Serum, plasma, cell culture supernatant and other biological samples.

Species Reactivity

Human

Detection Method

iELISA

Intended Use

In vitro quantitative determination of EPO-Ab concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

1. ELISA Microplate(Dismountable), 8×12, Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C. Stored for 6 month at -20°C
2. Lyophilized Standard, 2vial, Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C. Stored for 6 month at -20°C
3. HRP-labeled Antibody (Concentrated, 100×), 120ul, 2-8°C (Avoid Direct Light)
4. TMB Substrate, 10ml, 2-8°C (Avoid Direct Light)
5. Sample Dilution Buffer, 20ml, 2-8°C
6. Antibody Dilution Buffer, 10ml, 2-8°C
7. Stop Solution, 10ml, 2-8°C
8. Wash Buffer(25×), 30ml, 2-8°C
9. Plate Sealer, 5 pieces
10. Product Description, 1 copy
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Performance Characteristics

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Detection Range

0.781-50ng/ml

Sensitivity

0.469ng/ml

General Description

Anti-erythropoietin (anti-EPO) antibody-mediated pure red cell aplasia (PRCA) is a rarely seen disease, Pure red cell aplasia defined as severe anemia secondary to the virtual absence of red blood cell precursors in the bone marrow. The treatment against anti-EPO antibody-mediated PRCA included discontinuation of rHuEPO, immunosuppressive agents, intravenous immunoglobulin, plasmapheresis, or kidney transplantation.

Standard Curve

1. Calculate the mean OD450 value (using the original OD450 value or the corrected OD450 value) of the duplicate readings for each standard, control, and sample. Then, obtain the value of calculation by subtracting the OD450 blank.
2. Create a four parameter logistic curve by plotting the mean absorbance for each standard on the y -axis against the concentration on the x-axis. (Remove the OD450 blank during plotting.) Alternatively, you can use the curve fitting software offered by the microplate reader.
3. Calculate the sample concentration by substituting OD450 value into the standard curve. Diluted samples should be multiplied by the relevant dilution ratio.