EPO(Erythropoietin) ELISA Kit

Sample

Serum

Species Reactivity

Human

Detection Method

sELISA

Intended Use

The EPO ELISA is intended for the quantitative determination of Erythropoietin (EPO) in human serum.

Contents of Kit

Storage

Store all kit components at 2-8°C except the Wash Concentrate.

Performance Characteristics

Accuracy
Eighty five (85) samples, with EPO values ranging from 3.8 to 304 mIU/mL, were assayed by the CD ELISA procedure and an ELISA EPO kit. Linear regression analysis gives the following statistics:
CD ELISA = 0.94 ELISA Kit – 0.41 mIU/mL r = 0.989 N = 85
High Dose Hook Effect
The EPO ELISA kit has exhibited no "high dose hook effect" in standard diluent spiked with 200,000 mIU/mL of EPO. Additionally, three samples with known high EPO values (1,920 mIU/mL, 1,520 mIU/mL, and 966 mIU/mL) were tested without dilution and their results read much greater than the highest standard. Samples with EPO levels greater than the highest calibrator, however, should be diluted and re-assayed for correct values.

Precision

The Intra-assay precision of the EPO ELISA Test was calculated from 22 replicate determinations on each of the two samples.
Intra-Assay Variation

The inter-assay precision of the EPO ELISA Test was calculated from data on two samples obtained in 22 different assays.
Inter-Assay Variation

Sensitivity

The sensitivity, or minimum detection limit, of this assay is defined as the smallest single value, which can be distinguished from zero at the 95% confidence limit.
The EPO ELISA has a calculated sensitivity of 1.2 mIU/mL when using Calibrator B as the lowest calibrator. Hence, sample results below 1.2 mIU/mL should be reported as "Less than 1.2 mIU/mL".
The EPO ELISA with Calibrator G and Calibrator H has a calculated sensitivity of 0.6 mIU/mL Hence, sample results below 0.6 mIU/mL should be reported as "Less than 0.6 mIU/mL".