The rat monoclonal antibody named Epoclonalan reacts with human erythropoietin. Spleen cells from ACI strain rats that had been immunised with human urinary erythropoietin have been fused with Sp2/0 Ag-14 to produce this cell line. The alpha, beta and asialo forms of human erythropoietin are bound by the antibody to the same extent.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere and maintain cultures between 1-10x10^5 cells/ml.
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